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Series GSE15655 Query DataSets for GSE15655
Status Public on Apr 14, 2009
Title Baxter_etal_esb1_mapping
Organism Arabidopsis thaliana
Experiment type Genome variation profiling by array
Genome variation profiling by genome tiling array
Summary ABSTRACT: Background: Though central to our understanding of how roots perform their vital function of scavenging water and solutes from the soil, no direct genetic evidence currently exists to support the foundational model that suberin acts to form a chemical barrier limiting the extracellular, or apoplastic, transport of water and solutes in plant roots.
Methodologies/Principle Findings: Using the newly characterized enhanced suberin1 (esb1) mutant, we established a connection in Arabidopsis thaliana between suberin in the root, and both water movement through the plant, and solute accumulation in the shoot. Esb1 mutants, characterized by increased root suberin, were found to have reduced day time transpiration rates, and increased water use efficiency during their vegetative growth period. Furthermore, these changes in suberin and water transport were associated with decreases in the accumulation of Ca, Mn and Zn, and increases in the accumulation of Na, S, K, As, Se and Mo in the shoot.
Conclusions/Significance: Here we present direct genetic evidence establishing that suberin in the roots plays a critical role in controlling both water and mineral ion uptake and transport to the leaves. The changes observed in the elemental accumulation in leaves are also interpreted as evidence that a significant component of the radial root transport of Ca, Mn and Zn occurs in the apoplast.

Keywords: genomic hybridization bulked segregant analysis
 
Overall design Hybridizations from a set of Bulk Segregant analysis. An F2 population from 14501 in the col-0 background crossed to Ler-0 was analyzed. This series contains the 3 hybs from Ler used to identify Single Feature Polymorphisms, the 3 hybs of Col-0 they were compared to, and 1 hyb for each pool from the BSA mapping (High Ca/B pool, low Ca/B Pool). It also included 2 tiling array hybs: a wt (Col-0) and a mutant (esb1-1).
 
Citation(s) 19461889, 21421810
Submission date Apr 13, 2009
Last update date Aug 28, 2018
Contact name Ivan Baxter
E-mail(s) ibaxter@danforthcenter.org
Organization name USDA-ARS
Department PLant Genetics Research Unit
Lab Baxter
Street address 975 North Warson Rd
City St. Louis
State/province MO
ZIP/Postal code 63132
Country USA
 
Platforms (2)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
GPL10978 [At35b_MF] Affymetrix Arabidopsis Tiling 1.0F Array
Samples (10)
GSM143303 col3DNA
GSM143304 col1DNA
GSM143305 col2DNA
Relations
BioProject PRJNA116675

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE15655_RAW.tar 71.4 Mb (http)(custom) TAR (of CEL)
Processed data not provided for this record

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