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Series GSE156580 Query DataSets for GSE156580
Status Public on Aug 21, 2020
Title Intersectional analysis of chronic mild stress-induced lncRNA-mRNA interaction networks in rat hippocampus reveals potential anti-depression/anxiety drug targets
Organism Rattus norvegicus
Experiment type Expression profiling by array
Non-coding RNA profiling by array
Summary Despite studies providing insight into the neurobiology of chronic stress, depression and anxiety, long noncoding RNA (lncRNA)-mediated mechanisms underlying the common and distinct pathophysiology of these stress-induced disorders remain nonconclusive. In a previous study, we used the chronic mild stress paradigm to separate depression-susceptible, anxiety-susceptible and insusceptible rat subpopulations. In the current study, lncRNA and messenger RNA (mRNA) expression was comparatively profiled in the hippocampus of the three stress groups using microarray technology. Groupwise comparisons identified distinct sets of lncRNAs and mRNAs associated with the three different behavioral phenotypes of the stressed rats. To investigate the regulatory roles of the dysregulated lncRNAs upon mRNA expression, correlations between the differential lncRNAs and mRNAs were first analyzed by combined use of weighted gene coexpression network analysis and ceRNA theory-based methods. Subsequent functional analysis of strongly correlated mRNAs indicated that the dysregulated lncRNAs were involved in various biological pathways and processes to specifically induce rat susceptibility or resiliency to depression or anxiety. Further intersectional analysis of phenotype-associated and drug-associated lncRNA-mRNA networks and subnetworks assisted in identifying 16 hub lncRNAs as potential targets of anti-depression/anxiety drugs. Collectively, our study established the molecular basis for understanding the similarities and differences in pathophysiological mechanisms underlying stress-induced depression or anxiety and stress resiliency, revealing several important lncRNAs that represent potentially new therapeutic drug targets for depression and anxiety disorders.
 
Overall design After the tests, animals were decapitated, and the entire brain was rapidly removed. Then, we separated hippocampal tissues from the brain. After weighing the brains, we froze them rapidly in liquid N2 and stored them at a temperature of −80°C. Total RNA was extracted from tissues with TRIzol reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s instructions. Moreover, we measured RNA quality and quantity with a NanoDrop ND-1000. Then, we evaluated RNA integrity using standard electrophoresis.the hippocampus tissues were prepared for microarray data analysis
 
Contributor(s) Xiao M, Chen Y
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Submission date Aug 20, 2020
Last update date Aug 23, 2020
Contact name Jian Zhou
E-mail(s) zhoujian@cqmu.edu.cn
Phone +862368485763
Organization name Chongqing Medical University
Department Institute of Neuroscience
Street address 1 Yixueyuan Road, Yuzhong District
City Chongqing
State/province China
ZIP/Postal code 400016
Country China
 
Platforms (1)
GPL15690 Arraystar Rat LncRNA microarray (026403)
Samples (16)
GSM4734057 Hippocampus-cont-rep1
GSM4734058 Hippocampus-cont-rep2
GSM4734059 Hippocampus-cont-rep3
Relations
BioProject PRJNA658375

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE156580_RAW.tar 34.0 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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