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Status |
Public on May 05, 2009 |
Title |
Using high-density exon arrays to profile gene expression in closely related species (HJAY) |
Platform organism |
Homo sapiens |
Sample organisms |
Macaca mulatta; Pan troglodytes; Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Global comparisons of gene expression profiles between species provide significant insight into gene regulation, evolutionary processes, and disease mechanisms. In this work, we describe a flexible and intuitive approach for global expression profiling of closely related species, using high-density exon arrays designed for a single reference genome. The high-density probe coverage of exon arrays allows us to select the identical set of perfect-match probes for measuring expression levels of orthologous genes. This eliminates a serious confounding factor in probe affinity effects of species-specific microarray probes, and enables direct comparisons of estimated expression indexes across species.
Keywords: analysis of gene expression in tissues
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Overall design |
We conducted Affymetrix Human Exon Junction array (HJAY array) profiling of RNAs from human, chimpanzee and rhesus macaque cerebellums, with three replicates per species.
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Contributor(s) |
Xing Y |
Citation(s) |
19474342, 20864445 |
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Submission date |
Apr 14, 2009 |
Last update date |
Nov 05, 2019 |
Contact name |
Yi Xing |
E-mail(s) |
yi-xing@uiowa.edu
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Phone |
319-384-3099
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Fax |
319-384-3150
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Organization name |
University of Iowa
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Department |
Internal Medicine, Biomedical Engineering
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Street address |
3294 CBRB, 285 Newton Rd
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City |
Iowa City |
State/province |
IA |
ZIP/Postal code |
52242 |
Country |
USA |
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Platforms (1) |
GPL8444 |
Affymetrix GeneChip Human HJAY Array 17K |
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Samples (9)
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GSM392147 |
Human cerebellum, pooled, technical rep 1 |
GSM392148 |
Human cerebellum, pooled, technical rep 2 |
GSM392149 |
Human cerebellum, pooled, technical rep 3 |
GSM392150 |
Chimpanzee cerebellum, biological rep 1 |
GSM392151 |
Chimpanzee cerebellum, biological rep 2 |
GSM392152 |
Chimpanzee cerebellum, biological rep 3 |
GSM392153 |
Rhesus macaque cerebellum, biological rep 1 |
GSM392154 |
Rhesus macaque cerebellum, biological rep 2 |
GSM392155 |
Rhesus macaque cerebellum, biological rep 3 |
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This SubSeries is part of SuperSeries: |
GSE15666 |
Using high-density exon arrays to profile gene expression in closely related species |
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Relations |
BioProject |
PRJNA122927 |
Supplementary file |
Size |
Download |
File type/resource |
GSE15665_RAW.tar |
235.8 Mb |
(http)(custom) |
TAR (of CEL) |
Processed data included within Sample table |
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