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Series GSE156709 Query DataSets for GSE156709
Status Public on Feb 24, 2022
Title Robust Expansion of Human Pancreatic Progenitors by Small Molecules [RNA-seq]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary An unlimited source of functional human pancreatic β cells are in highly demand. Even with recent advances in pancreatic β-like cell differentiation from human pluripotent stem cells (hPSCs), several hurdles obviously remain and the differentiation protocols need to be further improved. Chemical strategies are particularly useful to address these challenges. Here, through chemical screening, we unexpectedly identified that BET bromodomain inhibitor I-BET151 could robustly promote the expansion of PDX1 and NKX6.1 double-positive human pancreatic progenitors (PPs). These hPSC-derived expandable pancreatic progenitors (ePPs) can proliferate extensively in a chemically defined condition with I-BET151. Even after long-term expansion, these ePPs maintain pancreatic progenitor cell status. In addition, ePPs can efficiently differentiate into pancreatic β-like cells (ePP-β cells). These ePP-β cells are functional and demonstrate glucose-stimulation insulin-secretion (GSIS) capacity. Mechanistically, I-BET151 can activate Notch signaling and promote the expression of key pancreatic progenitor-associated genes and transcriptional network. Conclusively, our studies achieve the long-term goal of robust expansion of human pancreatic progenitors and represent a significant step towards unlimited supplies of functional human pancreatic β cells that are of great interest for biomedical research and regenerative medicine.
 
Overall design RNA-seq of cells at defined developmental stages from stem cells to pancreatic β-like cells.RNA-seq of ePP-P9, ePP-P21 and ePP-derived pancreatic β-like cells. RNA-seq of PPs treated with DMSO or I-BET151.
 
Contributor(s) Ma X, Lu Y, Zhou Z, Zhu S
Citation(s) 35196077
Submission date Aug 23, 2020
Last update date Feb 25, 2022
Contact name Yunkun Lu
Organization name Zhejiang University
Department Life Sciences Institute
Street address 866 Yuhangtang Rd.
City Hangzhou
State/province Zhejiang
ZIP/Postal code 310058
Country China
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (14)
GSM4742939 hPSC
GSM4742940 DE
GSM4742941 PP2
This SubSeries is part of SuperSeries:
GSE156712 Robust Expansion of Human Pancreatic Progenitors by Small Molecules
Relations
BioProject PRJNA658807
SRA SRP278531

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE156709_RAW.tar 4.2 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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