|
| Status |
Public on Jan 06, 2021 |
| Title |
DNA methyation of intragenic CpG islands are required for differentiation from iPSC to NPC |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
|
| Summary |
We generated whole genome bisulfite sequencing (WGBS) data with high sequencing depth in induced pluripotent stem cell (iPSC), neuronal progentior cell (NPC) and Early Neuron (EN), and investigated the relationship between DNA methylation changes in CpG islands (CGIs) and corresponding gene expression during NPC differentiation
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| |
|
| Overall design |
WGBS for iPSC, NPC and EN. mRNA-seq for iPSC and NPC. H3K4me3 ChIP-seq for iPSC and NPC. H3K27me3 ChIP-seq for iPSC and NPC
|
| |
|
| Contributor(s) |
Kim Y, Choi W |
| Citation(s) |
33293480 |
| |
| Submission date |
Aug 24, 2020 |
| Last update date |
Jan 06, 2021 |
| Contact name |
Young-Joon Kim |
| E-mail(s) |
yjkim@yonsei.ac.kr
|
| Organization name |
Yonsei university
|
| Street address |
Yonsei-ro 50
|
| City |
Seoul |
| ZIP/Postal code |
03722 |
| Country |
South Korea |
| |
|
| Platforms (3) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL20795 |
HiSeq X Ten (Homo sapiens) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (13)
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|
| Relations |
| BioProject |
PRJNA658906 |
| SRA |
SRP278588 |
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