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Series GSE156768 Query DataSets for GSE156768
Status Public on Sep 30, 2023
Title Effect of PCIF1 knockdown on gene exppression in HeLa S3 cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We previously identified PCIF1 (Phosphorylated CTD Interacting Factor 1) as a novel phosphorylated C-terminal domain (CTD) of RNA polymerase II. We also recently identified PCIF1 as a new cap-specific adenine N6 methyltransferase (CAPAM) responsible for creating N6, 2’-O-dimethyladenosine (m6Am) at the 5’-end of mRNAs. However, it remains unclear how PCIF1 regulates gene expression.
To identify genes whose expression levels are affected by PCIF1 knockdown in human cultured cells, we performed gene expression profiling by microarray analysis.
 
Overall design HeLa S3 cells were seeded on 6-well plates at 1.0 × 105 cell/well and cultured at 37°C 24 h before transfection. Cells were transfected with either negative control siRNA or siRNA specific to human PCIF1 gene using Lipofectamine RNAi MAX (ThermoFisher) and further cultured for 72 h at 37°C.
 
Contributor(s) Ishiguro H, Sugita A, Yanagisawa N, Ohkuma Y, Tabuchi Y, Hirose Y
Citation(s) 39451207
Submission date Aug 24, 2020
Last update date Dec 05, 2024
Contact name Yutaka Hirose
E-mail(s) yh620@pha.u-toyama.ac.jp
Phone 81-76-434-7541
Organization name University of Toyama
Department Graduate School of Medicine and Pharmaceutical Sciences
Lab Laboratory of Gene Regulation
Street address 2630 Sugitani
City Toyama
State/province Toyama
ZIP/Postal code 930-0194
Country Japan
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (6)
GSM4743691 HeLa S3 negative control siRNA_rep1
GSM4743692 HeLa S3 negative control siRNA_rep2
GSM4743693 HeLa S3 PCIF1 siRNA#1_rep1
Relations
BioProject PRJNA658999

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE156768_RAW.tar 28.4 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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