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| Status |
Public on Aug 13, 2021 |
| Title |
Transcriptional toxicity of imidazolium- and cholinium-based ionic liquids |
| Organism |
Daphnia magna |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Ionic Liquids are a broad group of salts with low melting points that can be specifically tuned for a broad range of applications. Despite being initially considered “green” solvents, their better environmental friendliness compared to traditional solvents has been increasingly challenged. In this study, we aimed to investigate the molecular effects of ILs exposure by using RNA-sequencing to study differential gene expression patterns. Thus, we exposed Daphnia magna to 1-ethyl-3-methylimidazolium chloride ([C2mim]Cl), 1-dodecyl chloride-3-methylimidazolium ([C12mim]Cl) and cholinium chloride ([Chol]Cl). Results suggest that the three ILs share several mechanisms of toxicity, including cellular membrane and cytoskeleton damage, oxidative stress, inhibition of antioxidant enzymes, mitochondrial affectation, changes in protein biosynthesis and energy production, DNA damage, and ultimately, programmed cell death and disease initiation. Overall, the dataset revealed that [C2mim]Cl and [C12mim]Cl were, respectively, the least and the most toxic ILs at the transcriptional level. Also, it is reinforced that [Chol]Cl is not devoid of environmental hazardous potential. Unique gene expression signatures could also be identified for each IL.
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| Overall design |
12 samples of whole-body mRNA profiles of 48-hours old Daphnia magna: control (3 replicates), exposure to 1-ethyl-3-methylimidazolium chloride (3 replicates), exposure to 1-dodecyl chloride-3-methylimidazolium (3 replicates) and exposure to cholinium chloride (3 replicates)
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| Contributor(s) |
Jeremias G, Jesus F, Ventura SM, Gonçalves FM, Asselman J, Pereira JL |
| Citation(s) |
33199138 |
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| Submission date |
Aug 24, 2020 |
| Last update date |
Aug 15, 2021 |
| Contact name |
Guilherme de Abreu Jeremias |
| E-mail(s) |
jeremias@ua.pt, gabreujeremias@gmail.com
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| Organization name |
University of Aveiro
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| Department |
Biology Department
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| Street address |
Campus Universitário de Santiago
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| City |
Aveiro |
| ZIP/Postal code |
3810-193 |
| Country |
Portugal |
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| Platforms (1) |
| GPL24325 |
Illumina HiSeq 4000 (Daphnia magna) |
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| Samples (12)
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| GSM4743700 |
1-ethyl-3-methylimidazolium chloride_rep1 - C2mimCl_R1 |
| GSM4743701 |
1-ethyl-3-methylimidazolium chloride_rep2 - C2mimCl_R2 |
| GSM4743702 |
1-ethyl-3-methylimidazolium chloride_rep3 - C2mimCl_R3 |
| GSM4743703 |
1-dodecyl chloride-3-methylimidazolium_rep1 - C12mimCl_R1 |
| GSM4743704 |
1-dodecyl chloride-3-methylimidazolium_rep2 - C12mimCl_R2 |
| GSM4743705 |
1-dodecyl chloride-3-methylimidazolium_rep3 - C12mimCl_R3 |
| GSM4743706 |
cholinium chloride_rep1 - CholCl_R1 |
| GSM4743707 |
cholinium chloride_rep2 - CholCl_R2 |
| GSM4743708 |
cholinium chloride_rep3 - CholCl_R3 |
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| Relations |
| BioProject |
PRJNA659001 |
| SRA |
SRP278637 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE156769_RAW.tar |
1.8 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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