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Series GSE157022 Query DataSets for GSE157022
Status Public on Dec 21, 2020
Title Extracellular Vesicles From Liver Progenitor Cells Downregulates Fibroblast Metabolic Activity and Increase the Expression of Immune-Response Related Molecules
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Extracellular vesicles are an important part of cell to cell crosstalk, and help to the acceptor cells to prepare for changes in their environment. MLP29 cells are mouse liver progenitor cells that release EVs loaded with cell fate signaling. In the current work, we have fed 3T3-L1 mouse fibroblast with those EVs, and then analyzed the cellular response by proteomics and transcriptomics. Results showed a downregulation of both proteins and transcripts related to proliferative and metabolic routes dependent on TGFb, and an increase on the ERBB2 inhibitor protein (ERBIN). An increase in the transcription of CXCL2, as well as the increase of ribosome biogenesis and interferon response molecules points toward the activation of immune system signaling, compatible with a response against tissue damage.
 
Overall design Approximately 2 million of 3T3-L1 cells were treated with the EVs obtained from 200 million of MLP29 cell line. On average, this represents 20 ug of protein (measured by Bradford) coming from 8.9*E10 particles (measured with Nanosight LM10) for treatment. In total 6 plates were treated, with three different preparations of EVs and 3 preparations of control (the very same procedure of EV isolation over a non conditioned-media, to rule out effects associated to the culture media itself), during 24 hours, in 25 mM HEPES-containing complete DMEM medium (contaminating vesicles were first removed by overnight centrifugation at 110000×g). At the end, plates were washed twice with PBS, raised with Tryple (GIBCO) and then divided equally for proteomic and transcriptomics analysis. An aliquot was employed to measure cell viability, higher than 90% in all the cases. The trypsin was removed after pellet the cells 1000xg 5 min, frozen in dry ice and stored at -80ºC until processing.
 
Contributor(s) Royo F, Azkargorta M, Lavin JL, Cortazar AR, Clos M, Gonzalez-Lopez M, Barcena L, Del Portillo H, Yañez-Mo M, Marcilla A, Borras F, Peinado H, Guerrero I, Váles-Gómez MM, Cereijo U, Sardon T, Aransay AM, Elortza F, Falcon-Perez JM
Citation(s)
  • Royo F, Azkargorta M, Lavin JL, Clos-Garcia M et al. Extracellular Vesicles From Liver Progenitor Cells Downregulates Fibroblast Metabolic Activity and Increase the Expression of Immune-Response Related Molecules. Front Cell Dev Biol 2020;8:613583. PMID: 33511119
Submission date Aug 27, 2020
Last update date Mar 22, 2021
Contact name Ana Maria Aransay
E-mail(s) amaransay@cicbiogune.es
Phone 0034944061325
Organization name CIC bioGUNE
Department Genome Analysis Platform
Street address Parque tecnologico de Bizkaia, Building 801-A
City Derio
State/province BIZKAIA
ZIP/Postal code 48160
Country Spain
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (6)
GSM4751007 Control group [bg1]
GSM4751008 Control group [bg2]
GSM4751009 Control group [bg3]
Relations
BioProject PRJNA659799
SRA SRP279111

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE157022_JF_12_A_mRNAseq_ProcessedFile.tsv.gz 143.8 Kb (ftp)(http) TSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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