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Status |
Public on Feb 23, 2022 |
Title |
Transcriptome Profiling Provides Insights into Mongolian Gerbil as an Ideal NAFLD Model : Earlier Onset of Inflammation and Fibrosis as Well as High Expression of HCC-Associated Genes |
Organism |
Meriones unguiculatus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Mongolian gerbil (Meriones unguiculatus) is an ideal animal model for non-alcoholic fatty liver disease/nonalcoholic steatohepatitis (NAFLD/NASH) which is under development. Currently, the research and application of Mongolian gerbils have been largely limited due to the lack of genomic and transcriptome information. In the present study, we sought to better understand the underlying mechanism of the rapid development of NASH in high-fat diet (HFD)-fed Mongolian gerbils by analyzing the transcriptome profilings of different modeling times. Eight hub genes CD44, APP, CDC42, CD68, CXCR4, CSF1R, ADGRE1 and FERMT3 were obtained, which are all involved in inflammation response and CDC42 and FERMT3 are associated with hepatocellular carcinoma (HCC). Most of the hub genes showed high expression levels in the first stage of modeling-building (HFD for 4 weeks). We observed that feeding HFD for 4 weeks is sufficient to induce high expression of pro-fibrotic factors such as PDGFB and CTGF and to form collagen deposits. Intriguingly, we found four significant independent prognostic factors for HCC: GPC1, ARPC1B, DAB2 and CFL exhibiting high expression levels during the modeling time. Our findings indicated that, Mongolian gerbil is an ideal NAFLD model with rapid progression in inflammation and fibrosis. Prolonging the modeling time may lead to the formation of NAFLD-related HCC and further research is needed to investigate this possibility.
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Overall design |
18 male gerbils were randomly divided into two groups, namely normal feeding diet (ND) group and high-fat diet (HFD) group. Each group was divided into three time points of 4-week (4W), 8-week (8W) and 16-week (16W), with 3 gerbils in each group. The gerbils in ND group were fed with normal feed and had water freely. The gerbils in HFD group were fed with high-fat diet and had water freely.
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Contributor(s) |
Hong W, Zhang T, Yan J, Yu J, He B, Wu L, Chen Z |
Citation(s) |
34775841 |
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Submission date |
Aug 31, 2020 |
Last update date |
Feb 23, 2022 |
Contact name |
Chen Zhiyun |
E-mail(s) |
jcyjzxchen@163.com
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Phone |
86-571-86008657
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Organization name |
The First Affiliated Hospital of Zhejiang Chinese Medical University
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Street address |
No. 9, Street 9, Xiasha economic development zone, Jianggan district
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City |
Hangzhou |
ZIP/Postal code |
310016 |
Country |
China |
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Platforms (1) |
GPL29087 |
Illumina HiSeq 4000 (Meriones unguiculatus) |
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Samples (18)
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Relations |
BioProject |
PRJNA660357 |
SRA |
SRP279418 |
Supplementary file |
Size |
Download |
File type/resource |
GSE157169_Normalized_expression_levels_of_Gerbils.xlsx |
2.3 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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