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Series GSE157505 Query DataSets for GSE157505
Status Public on Jan 14, 2022
Title RNAseq for Identification of Doxycycline transcriptomic impact in N2 & CB4856 C. elegans worms
Organism Caenorhabditis elegans
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: Transcriptomic exploration for the identification of genes affected upon Doxycycline treatment in each of the two different worm strains studied
 
Overall design Design: Wild-type C. elegans (N2 Bristol and CB4856 Hawaii) were received from Andersen’s group. Bacterial strain E. coli OP50 and E. coli HT115 were obtained from the Caenorhabditis Genetic Center (CGC). Worms of each strain were cultured on plates seeded with E. coli OP50, then eggs were obtained by alkaline hypochlorite treatment of gravid adults. A synchronized L1 population was obtained by incubating the egg suspension in M9 butter overnight at room temperature. Approximately 2000 L1 worms were transferred onto plates with or without 15 μg/mL Dox seeded with E. coli HT115. L4 worms were harvested after 50 h by three times of washing with M9 buffer. Tubes containing worm pellets were immediately submerged in liquid nitrogen for snap freezing and stored at -80ºC until use. Three biological replicates were collected for each condition.
 
Contributor(s) Gao A, El Alam G, Hof K
Citation(s) 35118355
Submission date Sep 04, 2020
Last update date Feb 08, 2022
Contact name Gaby El Alam
E-mail(s) gaby.elalam@epfl.ch
Organization name École polytechnique fédérale de Lausanne
Department SV LISP
Lab Laboratory of Integrative Systems Physiology
Street address Station 15
City Lausanne
State/province Vaud
ZIP/Postal code 1015
Country Switzerland
 
Platforms (1)
GPL25145 BGISEQ-500 (Caenorhabditis elegans)
Samples (12)
GSM4768104 C. elegans N2 Control 1
GSM4768105 C. elegans N2 Control 2
GSM4768106 C. elegans N2 Control 3
Relations
BioProject PRJNA661536
SRA SRP280124

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Supplementary file Size Download File type/resource
GSE157505_RAW.tar 2.9 Mb (http)(custom) TAR (of TAB)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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