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| Status |
Public on Sep 01, 2021 |
| Title |
Comparative polyA+ RNA sequencing of hematopoietic stem cells from adult Osmr gene knock-out and wild-type mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Germinal deletion of the Osmr gene encoding the oncostatin M receptor alpha chain enhances the mobilization of hematopoietic stem cells in response to G-CSF and clinically relevant combinations of G-CSF with CXCR4 antagonist Plerixafor or cyclophosphamide. This effect is inderectly mediated by hematopoietic stem cell niches in the bone marrow because hematopoietic cells do not express the Osmr gene whereas bone marrow endothelial cells and mesenchymal cells forming niches do. We find tha tOsmr deletion in the niche increases hematopoietic stem cells chemotaxis to CXCL12 and quiescence while decreasing E-selectin expression on bone marrow endothelial cells.
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| Overall design |
To gain further insights on how absence of OSMR signaling in niches modulate HSC function, we sorted 1,000 lineage-negative KIT+ SCA1+ FLT3- CD48- cells, which include long-term and short-term reconstituting hematopoietic stem cells, from the bone marrow of adult naïve Osmr-/- mice and wild-type C57BL/6 control mice. PolyA+ RNA was isolated and sequenced. Each reaction is from 1,000 cells each from a different mouse (6 WT miCe and 5 Osmr-/- mice). Subsequent gene set enrichment analysis (GSEA) on Hallmark and Gene Ontology (GO) pathways were performed and genes of interest were validated using real time quantitative PCR.
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| Contributor(s) |
Bisht K, Levesque J, Tseng H |
| Citation(s) |
34518644 |
| |
| Submission date |
Sep 07, 2020 |
| Last update date |
Dec 01, 2021 |
| Contact name |
Jean-Pierre Levesque |
| E-mail(s) |
jp.levesque@mater.uq.edu.au
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| Organization name |
Mater Research Institute-University of Queensland
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| Department |
Mater Research Institute
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| Lab |
Levesque lab
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| Street address |
37 Kent St
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| City |
Brisbane |
| State/province |
Queensland |
| ZIP/Postal code |
4102 |
| Country |
Australia |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA662040 |
| SRA |
SRP280342 |
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