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| Status |
Public on Sep 08, 2020 |
| Title |
Identification of genes regulated by Fer2 in PAM neurons using an overexpression approach |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The transcription factor Fer2 plays a neuroprotective role in the dopaminergic neurons of the fly brain, but the underlying molecular mechanisms are not understood. By performing RNA-seq on isolated dopaminergic PAM neurons after Fer2 constitutive overexpression, we identified over one hundred genes differentially regulated in response to PAM-specific Fer2 overexpression.
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| Overall design |
RNA-seq on isolated dopaminergic PAM neurons from 14 day-old flies overexpressing a UAS-Fer2::FLAG transgene driven by a PAM specific GAL4 (R58-GAL4). Flies not carrying the UAS-Fer2::FLAG transgene were used as controls. N=3 per group.
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| Contributor(s) |
Loncle N, Nagoshi E |
| Citation(s) |
35301315 |
| |
| Submission date |
Sep 07, 2020 |
| Last update date |
Apr 06, 2022 |
| Contact name |
Federico Miozzo |
| Organization name |
University of Geneva
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| Street address |
30 Quai Ernest-Ansermet, CH-1211
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| City |
Geneva |
| ZIP/Postal code |
1205 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL17275 |
Illumina HiSeq 2500 (Drosophila melanogaster) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA662077 |
| SRA |
SRP280352 |
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