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Series GSE157662 Query DataSets for GSE157662
Status Public on May 03, 2021
Title Cardiomyocyte heterogeneity in zebrafish development and regeneration
Organism Danio rerio
Experiment type Expression profiling by high throughput sequencing
Summary Contrary to adult mammals, zebrafish are able to regenerate their heart after cardiac injury. This regenerative response relies, in part, on the endogenous ability of cardiomyocytes (CMs) to dedifferentiate and proliferate to replenish the lost muscle. However, CM heterogeneity and population dynamics during development and regeneration remain poorly understood. Through comparative transcriptomic analyses of the developing and adult zebrafish heart, we identified tnnc2 and tnni4b.3 expression as markers for CMs at early and late developmental stages, respectively. Using newly developed reporter lines for these genes, we investigated their expression dynamics during development and regeneration, and observed interesting expression patterns. tnnc2 reporter lines label most CMs at embryonic stages, and this labeling declines rapidly during larval stages; in adult hearts expression is only detectable in a subset of CMs. Conversely, expression of a tnni4b.3 reporter is initially visible in outer curvature CMs at larval stages, and it is subsequently present in a vast majority of the CMs in adult hearts. Interestingly, we find that the adult CMs labeled by the embryonic reporter display higher levels of Tg(tp1:EGFP) expression, which indicates active Notch signaling. To further characterize this CM population, we performed transcriptomic analysis and found that it expresses genes encoding components of the Notch signaling pathway as well as markers of immature CMs. Moreover, during heart regeneration, proliferating CMs in the injured area activate the embryonic CM reporter. Overall, our findings provide further evidence of cardiomyocyte heterogeneity in the adult zebrafish heart.
 
Overall design Total RNA was isolated from sorted myl7:EGFP+ (7000 cells) and tp1:EGFP+/myl7:DsRed2-NLS+ (2000 cells). In both cases, RNA was extracted using RNeasy Micro Kit (Qiagen) following manufacturer’s instructions.
 
Contributor(s) Taddese Tsedeke A, Allanki S, Jimenez-Amilburu V, Guenther S, Lai S, Stainier DY, Marín-Juez R
Citation(s) 33857482
Submission date Sep 08, 2020
Last update date Aug 02, 2021
Contact name Stefan Günther
E-mail(s) stefan.guenther@mpi-bn.mpg.de
Organization name MPI for heart and lung research
Street address ludwigtr. 43
City bad nauheim
ZIP/Postal code 61231
Country Germany
 
Platforms (1)
GPL20828 Illumina NextSeq 500 (Danio rerio)
Samples (4)
GSM4772980 myl7_1
GSM4772981 myl7_2
GSM4772982 tp1_1
Relations
BioProject PRJNA662347
SRA SRP281427

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE157662_counts.matrix.norm_anno.txt.gz 3.7 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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