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| Status |
Public on Sep 10, 2020 |
| Title |
Cranial manipulation modulates cholinergic pathway gene expression in an animal model of age-related cognitive decline |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this study, we examine the effect of cranial osteopathic manipulation (COM) on gene expression, in an animal model for age-related cognitive decline (aged rats). We found that COM significantly affected the expression of 36 genes in the neuronal pathway (False Discovery Rate (FDR) < 0.004). The top five neuronal genes with the largest fold-change (Slc5a7, Chat, Slc18a3, Adcy5 and Cacna2d2, >2-fold change, FDR<0.004) are part of the cholinergic neurotransmission mechanism, which is known to affect cognitive function.
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| Overall design |
Compare gene expression from RNA sequencing of tissue samples from prefrontal cortex of aged rats that have undergone cranial osteopathic manipulation to aged rats that did not receive the treatment
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| Contributor(s) |
Anandakrishnan R, Tobey H, Klein BG, Costa BM |
| Citation(s) |
34995434 |
| Submission date |
Sep 09, 2020 |
| Last update date |
Jan 29, 2022 |
| Contact name |
Ramu Anandakrishnan |
| E-mail(s) |
ramu@vt.edu
|
| Phone |
703-403-0234
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| Organization name |
Edward Via College of Osteopathic Medici
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| Department |
Biomedical Sciences
|
| Lab |
Molecular Mechanism of Disease
|
| Street address |
2265 Kraft Dr
|
| City |
Blacksburg |
| State/province |
VA |
| ZIP/Postal code |
24060 |
| Country |
USA |
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| Platforms (1) |
| GPL20084 |
Illumina NextSeq 500 (Rattus norvegicus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA662592 |
| SRA |
SRP281885 |
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