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Series GSE157770 Query DataSets for GSE157770
Status Public on Sep 11, 2020
Title Molecular signatures of immune cells and neuroblasts in the aged brain
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Normal aging is accompanied by escalating systemic inflammation. To comprehensively characterize the impact of aging on immune cells in the brain and periphery, we harvested immune cells from mouse spleen and brain tissues. Single cell sequencing was performed to compare the genetic signatures in these isolated immune cell subsets from spleen and brain tissues in young and aged mice. Our results demonstrate increased accumulations of immune cells such as natural killer (NK) cells in the aged brain as compared to the young brain. In addition, NK cells in the aged brain display augmented proliferation activity and cytotoxicity.  RNA-sequencing of neuroblasts isolated from the aged brain revealed that aging induces dysregulated expression of genes related to DNA damage response and upregulation of senescence signatures.
 
Overall design The 6 samples were divided into two groups to extract total RNA ,construct mRNA Library and use quantitative real-time PCR
 
Contributor(s) Jin W, Shi K, He W, Sun J, Van Kaer L, Shi F, Liu Q
Citation(s) 33257875
Submission date Sep 10, 2020
Last update date Dec 05, 2020
Contact name Wei-Na Jin
Organization name Beijing Tiantan Hospital, Capital Medical University
Department China National Clinical Research Center for Neurological Diseases
Street address NO.119, Southwest of the Fourth Ring Road, Fengtai District
City Beijing
State/province Beijing
ZIP/Postal code 100070
Country China
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (6)
GSM4774841 Y1: young sample 001
GSM4774842 Y2: young sample 002
GSM4774843 Y3: young sample 003
This SubSeries is part of SuperSeries:
GSE157772 Molecular signatures of immune cells and neuroblasts in the aged brain
Relations
BioProject PRJNA662714
SRA SRP281949

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE157770_Senescence.txt.gz 7.3 Kb (ftp)(http) TXT
GSE157770_cell_cycle.txt.gz 1.9 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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