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| Status |
Public on Sep 11, 2020 |
| Title |
Molecular signatures of immune cells and neuroblasts in the aged brain |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Normal aging is accompanied by escalating systemic inflammation. To comprehensively characterize the impact of aging on immune cells in the brain and periphery, we harvested immune cells from mouse spleen and brain tissues. Single cell sequencing was performed to compare the genetic signatures in these isolated immune cell subsets from spleen and brain tissues in young and aged mice. Our results demonstrate increased accumulations of immune cells such as natural killer (NK) cells in the aged brain as compared to the young brain. In addition, NK cells in the aged brain display augmented proliferation activity and cytotoxicity. RNA-sequencing of neuroblasts isolated from the aged brain revealed that aging induces dysregulated expression of genes related to DNA damage response and upregulation of senescence signatures.
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| Overall design |
The 4 samples were divided into two groups to sort single cell and construct single cell Library
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| Contributor(s) |
Jin W, Shi K, He W, Sun J, Van Kaer L, Shi F, Liu Q |
| Citation(s) |
33257875 |
| |
| Submission date |
Sep 10, 2020 |
| Last update date |
Dec 05, 2020 |
| Contact name |
Wei-Na Jin |
| Organization name |
Beijing Tiantan Hospital, Capital Medical University
|
| Department |
China National Clinical Research Center for Neurological Diseases
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| Street address |
NO.119, Southwest of the Fourth Ring Road, Fengtai District
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100070 |
| Country |
China |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE157772 |
Molecular signatures of immune cells and neuroblasts in the aged brain |
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| Relations |
| BioProject |
PRJNA662716 |
| SRA |
SRP281950 |
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