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Series GSE15780 Query DataSets for GSE15780
Status Public on Mar 01, 2011
Title Interplay between c-Jun and TAp73α/β contributes to the apoptosis-survival balance
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary The p53-family member p73 functions in various cellular signaling pathways and can have tumor suppressor properties. Several isoforms of p73 exist that differ considerably in their function. Whereas the functions of the N-terminal isoforms (TA and ΔNp73) and their opposing pro- and anti-apoptotic roles became evident, the functional differences of the distinct C-terminal spliceforms of TAp73 have remained unclear. Here, we characterized the genomic binding sites for TAp73α and TAp73β and identified a specific p73 consensus binding-motif. Furthermore, an AP1 motif is strongly enriched close to binding sites for TAp73α. These AP1 motif-containing target genes are selectively upregulated by TAp73α, while their mRNA expression is repressed upon TAp73β induction. Recruitment of c-Jun to the respective AP1 sites was impaired upon TAp73β expression in part due to downregulation of c-Jun. We show that several of these AP1-site containing TAp73α-induced genes reduce on apoptosis-induction suggesting an underlying molecular mechanism for the observed functional differences between TAp73α and TAp73β.
Overall design ChIP-seq and RNA-seq profiles of TAp73alpha, TAp73beta and p53 stably transfected in human osteosarcoma Saos cells
Contributor(s) Koeppel M, van Heeringen SJ, Kramer D, Smeenk L, Forkink M, Janssen-Megens EM, Stunnenberg HG, Lohrum M
Citation(s) 21459846
Submission date Apr 22, 2009
Last update date May 15, 2019
Contact name Marion Lohrum
Phone +31 24 3610541
Fax +31 24 3610520
Organization name NCMLS
Department Molecular Biology
Street address P.O. Box 9101
City Nijmegen
ZIP/Postal code 6500 HB
Country Netherlands
Platforms (1)
GPL9052 Illumina Genome Analyzer (Homo sapiens)
Samples (15)
GSM501686 ChIP_Input_control
GSM501687 TAp73alpha_ChIPSeq_replicate1
GSM501688 TAp73alpha_ChIPSeq_replicate2
BioProject PRJNA116853
SRA SRP001997

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Supplementary file Size Download File type/resource
GSE15780_RAW.tar 987.0 Mb (http)(custom) TAR (of BED)
GSE15780_README.txt 1.1 Kb (ftp)(http) TXT
GSE15780_Saos_TAp73alpha_peaks.bed.gz 132.7 Kb (ftp)(http) BED
GSE15780_Saos_TAp73beta_peaks.bed.gz 201.6 Kb (ftp)(http) BED
GSE15780_Saos_expression.tsv.gz 340.1 Kb (ftp)(http) TSV
GSE15780_Saos_p53_peaks.bed.gz 86.3 Kb (ftp)(http) BED
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Processed data provided as supplementary file
Processed data are available on Series record
Raw data are available in SRA

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