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Series GSE157821 Query DataSets for GSE157821
Status Public on May 02, 2024
Title Single cell RNA-seq of mouse HSC treated with iron chelator deferoxamine (DFO)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We characterized longer-term effects of iron homeostasis activation on hematopoietic stem cells by characterizing transcriptomic chagnes at the singe cell level 48hrs after iron chelatior treatement (vehicle treatemnt as controls).
 
Overall design For isolation of mouse HSC, bone marrow cells were isolated from tibiae, femurs, pelvic bones and vertebrae of wildtype C57/B6 mice at the age of 6 to 8-weeks. Lineage-depleted bone marrow cells from 8 mice were pooled and prospective HSC (Lin−Sca-1+c-Kit+CD150+CD48−) were sorted on MoFlo Astrios (Beckman Coulter). Thereafter, FACS-sorted HSC were exposed to iron chelator (DFO, Sigma) or vehicle (water) for 48 hours. Treated cells were collected and viability of >95% was determined by trypan blue exclusion. Twenty thousand (20,000) cells from each treatment group were then subjected to 10x Genomics platform for library preparation. Following quality control assessment with Agilent 2100 Bioanalyzer, approximately 50ng libraries were sequenced on BGISEQ-500 platform.
 
Contributor(s) Kao Y, Will B
Citation(s) 38402617
Submission date Sep 11, 2020
Last update date Aug 01, 2024
Contact name Britta Will
E-mail(s) britta.will@einsteinmed.edu
Organization name Albert Einstein College of Medicine
Department Department of Cell Biology
Lab Chanin 401
Street address 1300 Morris Park Ave
City Bronx
State/province NEW YORK
ZIP/Postal code 10461
Country USA
 
Platforms (1)
GPL23479 BGISEQ-500 (Mus musculus)
Samples (2)
GSM4775439 Mouse HSC - Control
GSM4775440 Mouse HSC - IC
Relations
BioProject PRJNA662917
SRA SRP282045

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Supplementary file Size Download File type/resource
GSE157821_RAW.tar 753.6 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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