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Status |
Public on Dec 15, 2010 |
Title |
Identification of new markers of cutaneous melanoma invasion |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
T1C3 is a clonal cell line from a human melanoma tumor, expressing PNA lectin, and characterized by a high capacity of dermal invasion. IC8 is a clonal cell line from a human melanoma tumor, negative for PNA lectin, and non-invasive. To identify new gene markers involved in the mechanisms of early invasion, oligonuclotide microarrays were used to assess transcriptional chnages between the two cell lines.
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Overall design |
Dye-swap hybridization were performed. Slides were scanned with a Genepix 4000 microarray scanner (Axon Instruments, Molecular devices, Sunnyvale, CA). For each hybridized spot, the Cy3 and Cy5 fluorescence values were obtained by using Genepix Pro 4.0 software (Axon Instruments) and were saved as a result file.
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Contributor(s) |
Lamartine J, Berthier O, El Kharbili M, Casu L, Gidrol X, Amiot F |
Citation(s) |
21081927 |
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Submission date |
Apr 23, 2009 |
Last update date |
Mar 21, 2012 |
Contact name |
Jerome Lamartine |
E-mail(s) |
jerome.lamartine@univ-lyon1.fr
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Phone |
+33 4 72 72 26 66
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Organization name |
Université Lyon I
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Lab |
LBTI
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Street address |
7 passage du Vercors
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City |
LYON |
ZIP/Postal code |
F69367 |
Country |
France |
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Platforms (1) |
GPL4803 |
CEA - DSV/DRR/SGF : Human 25k oligo v1.0 |
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Samples (4)
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Relations |
BioProject |
PRJNA116829 |