|
Status |
Public on Apr 14, 2021 |
Title |
Impact of LXR activation on alternatively activated human macrophages |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
|
Summary |
To investigate how LXR activation potentiates expression of Th2 cytokine-dependent genes in primary human macrophages, we pulsed macrophages with synthetic LXR ligand T0901317 then polarized cells to alternatively activated macrophages with IL-4 or IL-13.
|
|
|
Overall design |
Human monocytes derived from three individual donors were differentiated into naïve macrophages with RPMI 1640 medium containing 5% AB-positive human serum for 7 days. Naive macrophages were pulsed with T0901317 (1 µM) for 3 hours, then media was aspirated and cells were polarized to alternatively activated macrophages with IL-4 (5 ng/ml) or IL-13 (10 ng/ml) for 24 hours.
|
|
|
Contributor(s) |
Snodgrass RG, Brüne B |
Citation(s) |
- Snodgrass RG, Benatzy Y, Schmid T, Namgaladze D et al. Efferocytosis potentiates the expression of arachidonate 15-lipoxygenase (ALOX15) in alternatively activated human macrophages through LXR activation. Cell Death Differ 2021 Apr;28(4):1301-1316. PMID: 33177619
|
|
Submission date |
Sep 16, 2020 |
Last update date |
Apr 14, 2021 |
Contact name |
Ryan G Snodgrass |
E-mail(s) |
snodgrass@biochem.uni-frankfurt.de
|
Organization name |
Goethe-University Frankfurt
|
Department |
Institute of Biochemistry I
|
Street address |
Theodor-Stern-Kai 7, building 74
|
City |
Frankfurt |
ZIP/Postal code |
60590 |
Country |
Germany |
|
|
Platforms (1) |
GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
Samples (18)
|
|
Relations |
BioProject |
PRJNA663777 |
SRA |
SRP282556 |