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Series GSE158022 Query DataSets for GSE158022
Status Public on Apr 14, 2021
Title Impact of LXR activation on alternatively activated human macrophages
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary To investigate how LXR activation potentiates expression of Th2 cytokine-dependent genes in primary human macrophages, we pulsed macrophages with synthetic LXR ligand T0901317 then polarized cells to alternatively activated macrophages with IL-4 or IL-13.
 
Overall design Human monocytes derived from three individual donors were differentiated into naïve macrophages with RPMI 1640 medium containing 5% AB-positive human serum for 7 days. Naive macrophages were pulsed with T0901317 (1 µM) for 3 hours, then media was aspirated and cells were polarized to alternatively activated macrophages with IL-4 (5 ng/ml) or IL-13 (10 ng/ml) for 24 hours.
 
Contributor(s) Snodgrass RG, Brüne B
Citation(s) 33177619
Submission date Sep 16, 2020
Last update date Apr 14, 2021
Contact name Ryan G Snodgrass
E-mail(s) snodgrass@biochem.uni-frankfurt.de
Organization name Goethe-University Frankfurt
Department Institute of Biochemistry I
Street address Theodor-Stern-Kai 7, building 74
City Frankfurt
ZIP/Postal code 60590
Country Germany
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (18)
GSM4785378 untreated macrophages rep1
GSM4785379 untreated macrophages rep2
GSM4785380 untreated macrophages rep3
Relations
BioProject PRJNA663777
SRA SRP282556

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE158022_Con_vs_IL13.txt.gz 814.7 Kb (ftp)(http) TXT
GSE158022_Con_vs_IL4.txt.gz 817.2 Kb (ftp)(http) TXT
GSE158022_RAW.tar 3.4 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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