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Status |
Public on Feb 03, 2021 |
Title |
Analysis of chromatin accessibility in adult mouse pituitary using single-cell ATAC-seq |
Organism |
Mus musculus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
To gain insight into patterns of chromatin accessibility in distinct cell subtypes of mouse pituitary, pituitaries from two adult mice were collected and subjected to single-cell ATAC-seq analysis using 10X Genomics platform
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Overall design |
Two adult pituitaries were dissected and processed indiviudally for single-cell ATAC-seq. Nuclei were isolated and diluted to achieve ~6000 individual nuclei for library construction. Libraries were sequenced to ~50% saturation. Analysis was done using Cell Ranger pipeline.
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Contributor(s) |
Shen J, Xu Q, Krawczyk M, Skowronska-Krawczyk D |
Citation(s) |
33009549 |
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Submission date |
Sep 16, 2020 |
Last update date |
Feb 03, 2021 |
Contact name |
Michal Krawczyk |
E-mail(s) |
mikrawczyk@gmail.com
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Organization name |
UCSD
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Department |
Ophthalmology
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Street address |
9500 Gilman Dr
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City |
La Jolla |
ZIP/Postal code |
92093 |
Country |
USA |
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Platforms (1) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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Samples (2) |
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Relations |
BioProject |
PRJNA663883 |
SRA |
SRP282610 |