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| Status |
Public on Sep 27, 2022 |
| Title |
Development of Xenopus Mucociliary Epithelium |
| Organism |
Xenopus laevis |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Here we profile 10 distinct Xenopus MCE developmental stages throughout embryogenesis and characterise the expression patterns and state transition accompanying cell-fate decisions.
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| Overall design |
We harvest holoblastic blastula stage embryos (Nieuwkoop and Faber, NF stage 8), culture the explants ex vivo (referred as organoids), and profile 10 MCE development stages spanning blastula (stage 8) to gastrulation (stage 10.5, 12), neurula (stage 13, 16, 18, 21) and early tailbud development (stage 22, 24, 27), (22, 23) . After quality control (doublet removal and normalization; methods), we obtain 33,990 single-cell transcriptomes with high sampling and representation across all developmental stages.
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| Contributor(s) |
Møller AF, Lee J, Chae S, Kwon T, Sedzinski J, Natarajan KN |
| Citation(s) |
37027470 |
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| Submission date |
Sep 16, 2020 |
| Last update date |
May 02, 2023 |
| Contact name |
Taejoon Kwon |
| E-mail(s) |
tkwon@unist.ac.kr
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| Organization name |
Ulsan National Institute of Science and Technology
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| Department |
Department of Biomedical Engineering
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| Street address |
50 Unist-gil
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| City |
Ulsan |
| ZIP/Postal code |
44919 |
| Country |
South Korea |
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| Platforms (1) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA663910 |
| SRA |
SRP282633 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE158088_RAW.tar |
1.7 Gb |
(http)(custom) |
TAR (of TAR) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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