|
| Status |
Public on Jan 14, 2022 |
| Title |
Redundant mechanisms driven independently by RUNX1 and GATA2 for hematopoietic development |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Here we used RNA sequencing to characterize the transcriptional profile of the total RNA from c-Kit+ cells of Runx1 KO and control mice.
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| |
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| Overall design |
For bulk RNA-sequencing: Total RNA from c-Kit+ cells was extracted with AllPrep DNA/RNA/Protein Mini Kit (QIAGEN). Poly-A selected stranded mRNA libraries were constructed using the Illumina TruSeq Stranded mRNA Sample Prep Kits according to manufacturer’s instructions. Unique barcode adapters were applied to each library. All libraries were combined in equimolar proportion into one pool for sequencing with a HiSeq2500 sequencer.
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| Web link |
https://pubmed.ncbi.nlm.nih.gov/34492681/
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| |
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| Contributor(s) |
Bresciani E, Kwon E, Yu K, dell'Orso S |
| Citation(s) |
34492681 |
| |
| Submission date |
Sep 16, 2020 |
| Last update date |
Jan 16, 2022 |
| Contact name |
Kai Yu |
| E-mail(s) |
kai.yu@nih.gov
|
| Organization name |
NHGRI
|
| Street address |
50 South Drive, Bldg 50, Room 5151
|
| City |
Bethesda |
| State/province |
Maryland |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE158101 |
Redundant mechanisms driven independently by RUNX1 and GATA2 for hematopoietic development |
|
| Relations |
| BioProject |
PRJNA663963 |
| SRA |
SRP282673 |
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