NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE158105 Query DataSets for GSE158105
Status Public on May 10, 2021
Title Foxp3 enhancers synergize to maximize regulatory T cell suppressive capacity [ATAC-Seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Treg cells bearing a diverse antigen receptor repertoire suppress pathogenic T cells and maintain immune homeostasis during their long lifespan. How their robust function is determined genetically remains elusive. Here, we investigate the regulatory space of the cis-regulatory elements of Treg lineage–specifying factor Foxp3. Foxp3 enhancers are known as distinct readers for environmental cues in promoting Treg cell induction or lineage stability. However, their single deficiencies cause mild, if any, immune dysregulation, leaving the key transcriptional mechanisms determining Foxp3 expression and thereby Treg suppressive capacity uncertain. We examined the collective activities of Foxp3 enhancers and found that they coordinate to maximize Treg cell induction, Foxp3 expression level, or lineage stability through distinct modes and that ablation of synergistic enhancers led to lethal autoimmunity in young mice. Thus, the induction and maintenance of a diverse, stable Treg cell repertoire rely on combinatorial Foxp3 enhancers, suggesting broad, stage-specific, synergistic activities of cell-intrinsic factors and -extrinsic cues in determining Treg suppressive capacity.
 
Overall design ATAC sequencing was used to assess the chromatin architecture in regulatory T cells and naïve CD4 T cells of wild-type and Foxp3 enhancer deficiencies.
 
Contributor(s) Xu B, Li J, Zong X, Feng Y
Citation(s) 34086055
Submission date Sep 17, 2020
Last update date Aug 09, 2021
Contact name Beisi Xu
E-mail(s) beisi.xu@stjude.org
Organization name St Jude Children's Research Hosipital
Department Center for Applied Bioinformatics
Street address 262 Danny Thomas Pl
City Memphis
State/province Tennessee
ZIP/Postal code 38105
Country USA
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (12)
GSM4792256 ATAC-seq-regulatory T cells-deficiency of Foxp3 enhancer CNS2 (CNS2KO)
GSM4792257 ATAC-seq-regulatory T cells-wild type Foxp3-GFP
GSM4792258 ATAC-seq-regulatory T cells-deficiency of Foxp3 enhancer CNS0 and CNS2 (CNS02DKO)
This SubSeries is part of SuperSeries:
GSE158223 Foxp3 enhancers synergize to maximize regulatory T cell suppressive capacity
Relations
BioProject PRJNA664020
SRA SRP282818

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE158105_RAW.tar 330.6 Mb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap
External link. Please review our privacy policy.