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| Status |
Public on Feb 15, 2021 |
| Title |
Single-cell characterization of transcriptomic heterogeneity in lymphoblastoid cell lines |
| Organisms |
Homo sapiens; human gammaherpesvirus 4 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report single-cell RNA sequencing datasets for three lymphoblastoid cell lines (LCLs) prepared by infection of primary B cells with Epstein-Barr Virus (EBV). Inter- and intra-sample transcriptional variance is observed with respect to immunoglobulin heavy chain class; host gene sets involved in survival, proliferation and differentiation; and viral replication state. We propose that the observed heterogeneity arises from the dynamic nature of host-pathogen interactions, donor-specific compositions of primary B cells, and other experimental factors such as stochastic clonal evolution in cell culture conditions.
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| Overall design |
Characterization of three different lymphoblastoid cell lines. Two samples (LCL_777_B958 and LCL_777_M81) were derived from the same donor and infected with different EBV strains (B95-8 and M81). A third sample (LCL_461_B958) was derived from a different donor and infected with B95-8.
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| Contributor(s) |
SoRelle ED, Dai J, Zhou JY, Giamberardino SN, Bailey JA, Gregory SG, Chan C, Luftig MA |
| Citation(s) |
33501914, 36248899 |
| |
| Submission date |
Sep 21, 2020 |
| Last update date |
Oct 28, 2022 |
| Contact name |
Elliott Daniel SoRelle |
| E-mail(s) |
elliott.sorelle@duke.edu
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| Organization name |
Duke University
|
| Department |
Molecular Genetics & Microbiology
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| Lab |
Micah Luftig Lab
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| Street address |
130 Research Drive (CARL Building)
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| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
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| Platforms (1) |
| GPL23362 |
Illumina HiSeq 4000 (Homo sapiens; Human gammaherpesvirus 4) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA664714 |
| SRA |
SRP284226 |
