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Series GSE158275 Query DataSets for GSE158275
Status Public on Feb 15, 2021
Title Single-cell characterization of transcriptomic heterogeneity in lymphoblastoid cell lines
Organisms Homo sapiens; human gammaherpesvirus 4
Experiment type Expression profiling by high throughput sequencing
Summary We report single-cell RNA sequencing datasets for three lymphoblastoid cell lines (LCLs) prepared by infection of primary B cells with Epstein-Barr Virus (EBV). Inter- and intra-sample transcriptional variance is observed with respect to immunoglobulin heavy chain class; host gene sets involved in survival, proliferation and differentiation; and viral replication state. We propose that the observed heterogeneity arises from the dynamic nature of host-pathogen interactions, donor-specific compositions of primary B cells, and other experimental factors such as stochastic clonal evolution in cell culture conditions.
Overall design Characterization of three different lymphoblastoid cell lines. Two samples (LCL_777_B958 and LCL_777_M81) were derived from the same donor and infected with different EBV strains (B95-8 and M81). A third sample (LCL_461_B958) was derived from a different donor and infected with B95-8.
Contributor(s) SoRelle ED, Dai J, Zhou JY, Giamberardino SN, Bailey JA, Gregory SG, Chan C, Luftig MA
Citation(s) 33501914, 36248899
Submission date Sep 21, 2020
Last update date Oct 28, 2022
Contact name Elliott Daniel SoRelle
Organization name Duke University
Department Molecular Genetics & Microbiology
Lab Micah Luftig Lab
Street address 130 Research Drive (CARL Building)
City Durham
State/province NC
ZIP/Postal code 27710
Country USA
Platforms (1)
GPL23362 Illumina HiSeq 4000 (Homo sapiens; Human gammaherpesvirus 4)
Samples (3)
GSM4796271 LCL_777_B958
GSM4796272 LCL_777_M81
GSM4796273 LCL_461_B958
BioProject PRJNA664714
SRA SRP284226

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Supplementary file Size Download File type/resource
GSE158275_RAW.tar 94.1 Mb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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