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| Status |
Public on Mar 05, 2021 |
| Title |
Single-cell joint detection of chromatin occupancy and transcriptome enables higher-dimensional epigenomic reconstructions |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We develop CoTECH, a combinatorial barcoding method allowing for high-throughput parallel single-cell detection of chromatin occupancy and transcriptome.
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| |
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| Overall design |
Protein A-Tn5 was enriched through specific antibodies to genomic regions and Tn5 generated indexed chromatin. Following tagmentation, mRNA inside cells were captured by oligo dT barcode primers and reversed-transcribed. Combinatorial indexing strategy was implemented on both ChIPed DNA parts and RNA parts.
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| |
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| Contributor(s) |
Xiong H, Luo Y, Wang Q, He A |
| Citation(s) |
33958790 |
| Submission date |
Sep 23, 2020 |
| Last update date |
Jun 04, 2021 |
| Contact name |
Haiqing Xiong |
| E-mail(s) |
haiqingxiong@pku.edu.cn
|
| Organization name |
Peking University
|
| Street address |
5 Yiheyuan Road, Haidian District
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
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| Platforms (2) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (16491)
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| Relations |
| BioProject |
PRJNA665224 |
| SRA |
SRP285110 |
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