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| Status |
Public on Dec 07, 2020 |
| Title |
Transciption profiling of leptin receptor-expressing neurons in the Nucleus of the Solitary Tract |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
This study aims to identify and compare unique transcription profiles of neurons in the NTS that express the leptin receptor. We conducted single-cell patch RNA-sequencing experiments to classify neurons first by their unique firing properties and, second, transciptional profiles. We found two unique cellular populations: type1 and type2 distinguishable by firing patterns and transciption profile.
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| Overall design |
cytoplasmic and nuclear RNA were harvested from single-neurons of transgenic mice with yellow flourescent protein expressed in leptin receptor-expressin neurons (LepRb-Cre; Halo-eYFP)
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| Contributor(s) |
Do J, Martina M |
| Citation(s) |
33176139 |
| |
| Submission date |
Sep 25, 2020 |
| Last update date |
Dec 07, 2020 |
| Contact name |
Marco Martina |
| E-mail(s) |
m-martina@northwestern.edu
|
| Organization name |
Northwestern University
|
| Department |
Physiology
|
| Street address |
300 E Superior
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60611 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA665714 |
| SRA |
SRP285398 |
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