|
| Status |
Public on May 04, 2010 |
| Title |
Metabolism-based resistance to herbicides in black-grass |
| Platform organism |
Triticum aestivum |
| Sample organism |
Alopecurus myosuroides |
| Experiment type |
Expression profiling by array
|
| Summary |
Resistance to herbicides in weeds can be due to alteration(s) in the gene encoding the herbicide target site, or to herbicide degradation via a deviation in plant general metabolism. If target-site-based resistance is easy to study, the multigenic control of metabolism-based resistance renders it much more complex to study. Metabolism-based resistance to herbicides represents the major part of herbicide resistance in black-grass. Its most likely basis is an overexpression of genes encoding enzymes degrading herbicides. We thus seek to identify such overexpressed genes by comparing the transcriptomes of resistant and sensitive black-grass individuals belonging to an F2 line segregating for two resistance genes. Given there are no genomic tools developed for black-grass, this approach will use heterologous hybridisation onto a wheat Affymetrix microarray.
Comparison using heterologous hybridisation onto a wheat whole-genome microarray of transcriptome of three pools of black-grass plants obtained 2h30 after herbicide spraying at field rate. The three pools correspond to: · Sensitive plants (killed by herbicide). · Moderately resistant plants (growth impaired by herbicide but plants still alive) · Resistant plants (growth unimpaired by herbicide)
|
| |
|
| Overall design |
6 arrays - wheat
|
| Web link |
http://urgv.evry.inra.fr/CATdb
|
| |
|
| Contributor(s) |
Balzergue S, Délye C, Petit C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 28, 2009 |
| Last update date |
Dec 04, 2018 |
| Contact name |
sandrine balzergue |
| Organization name |
INRA
|
| Lab |
IRHS
|
| Street address |
Rue G. Morel
|
| City |
Beaucouze |
| ZIP/Postal code |
49000 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL3802 |
[wheat] Affymetrix Wheat Genome Array |
|
| Samples (6)
|
| GSM398307 |
S1 with array type wheat from Affymetrix |
| GSM398308 |
S2 with array type wheat from Affymetrix |
| GSM398309 |
R2 with array type wheat from Affymetrix |
| GSM398310 |
R1 with array type wheat from Affymetrix |
| GSM398311 |
petitr1 with array type wheat from Affymetrix |
| GSM398312 |
petitr2 with array type wheat from Affymetrix |
|
| Relations |
| BioProject |
PRJNA116967 |
Less...
More...