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Series GSE158653 Query DataSets for GSE158653
Status Public on Oct 13, 2021
Title Long-term metabolic effect of chronic variable stress on liver function and the role of FGF21
Organism Mus musculus
Experiment type Expression profiling by array
Summary Chronic stress disorders lead to metabolic complications, including hepatic lipid accumulation. Here we address the role of FGF21 in the hepatic metabolic regulation in a mouse model for chronic variable stress (Cvs). Global FGF21KO and WT mice show an intact stress response with short-term Cvs induced alterations in hepatic lipid metabolism, mitochondrial function and gene expression. Hepatic steatosis is found with the highest significance in enrichment analyses of hepatic transcriptome data, with PPARa and SREBP-1 as main upstream regulatory molecules, which are directly associated to FGF21. After 3 months recovery, stress-related metabolic improvements are not visible in FGF21KO mice in contrast to WT mice, indicating the crucial role of FGF21 in the post-stress metabolic adaptations. Overall, we suggest that Cvs-induced gene regulation determines the metabolic late effects after stress. Furthermore, FGF21 is a key player in the protection from stress-induced hepatic lipid accumulation.
 
Overall design Wildtype (WT; FGF21+/+) and Fgf21 knockout (FGF21KO; FGF21-/-) (doi:https://doi:10.1210/en.2009-0119) male mice were stressed with our Cvs protocol as previously described (doi:https://doi.org/10.1016/j.molmet.2018.06.012).or left untreated (Ctrl). Transcriptome analyses (MTA array, Affymetrix) was performed in Ctrl and Cvs liver biopsies of each genotype. Mice were phenotyped for body composition, serum analyses, physiological tolerance tests and mitochondrial function, both immediately after stress and after 3 month recovery to determine longitudinal metabolic stress adaptation and the role of FGF21 in this context.
 
Contributor(s) Dille M, Kotzka J, Knebel B
Citation(s) 34624498
Submission date Sep 28, 2020
Last update date Oct 14, 2021
Contact name Birgit Knebel
Organization name German Diabetes Center
Department Cinical Biochemistry and Pathobiochemistry
Street address Auf'm Hennekamp 65
City Duesseldorf
ZIP/Postal code 40225
Country Germany
 
Platforms (1)
GPL21877 [MTA-1_0] Affymetrix Mouse Transcriptome Array 1.0
Samples (20)
GSM4804972 254_KO_Ctrl_(MTA-1_0)
GSM4804973 255_KO_Ctrl_(MTA-1_0)
GSM4804974 291_KO_Ctrl_(MTA-1_0)
Relations
BioProject PRJNA666130

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE158653_RAW.tar 655.5 Mb (http)(custom) TAR (of CEL, CHP)
Processed data provided as supplementary file

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