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Status |
Public on Sep 29, 2020 |
Title |
Identification of genes with different expression in wild- and half-size Xenopus laevis embryos by transcriptome comparison |
Organism |
Xenopus laevis |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
To identify genes with different expression levels in Xenopus laevis embryos of different sizes, RNAseq of middle gastrula RNA of the wild-size and artificially prepared half-size embryos was done. As a result, several genes with drastically different expression between these two types of embryos were found.
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Overall design |
Three biological replicates were analyzed for 2 conditions (intact embryos vs their siblings divided at two-blastomere stage)
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Web link |
https://doi.org/10.1016/j.devcel.2021.11.021
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Contributor(s) |
Orlov E, Zaraisky A |
Citation(s) |
- Orlov EE, Nesterenko AM, Korotkova DD, Parshina EA et al. Targeted search for scaling genes reveals matrixmetalloproteinase 3 as a scaler of the dorsal-ventral pattern in Xenopus laevis embryos. Dev Cell 2022 Jan 10;57(1):95-111.e12. PMID: 34919801
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Submission date |
Sep 28, 2020 |
Last update date |
Jul 14, 2022 |
Contact name |
Evgeny E Orlov |
E-mail(s) |
Orlov_evgeny@yahoo.com
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Organization name |
Shemyakin-Ovchinnikov Institute of Bioorganic Chemstry, RAS
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Department |
Department of Genomics and Postgenomic Technologies
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Lab |
Laboratory of molecular bases of embryogenesis
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Street address |
Miklukho-Maklaya 16/10
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City |
Moscow |
ZIP/Postal code |
117997 |
Country |
Russia |
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Platforms (1) |
GPL18936 |
Illumina HiSeq 2500 (Xenopus laevis) |
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Samples (6)
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Relations |
BioProject |
PRJNA666158 |
SRA |
SRP285632 |