GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE158669 Query DataSets for GSE158669
Status Public on Apr 14, 2021
Title BCDIN3D RNA methyltransferase stimulates Aldolase C expression and glycolysis through let-7 microRNA in breast cancer cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Summary BCDIN3D is an RNA modifying enzyme that methylates specific precursor microRNAs and tRNAHis. In addition to breast cancer, BCDIN3D may also be linked to metabolism, as its gene locus is associated with obesity and T2D. In order to uncover metabolic pathways regulated by BCDIN3D in cancer, we performed an unbiased analysis of the metabolome, transcriptome, and proteome of breast cancer cells depleted for BCDIN3D. Intersection of these analyses showed that BCDIN3D-depleted cells have increased levels of Fructose 1,6 Bisphosphate (F1,6-BP), the last six-carbon glycolytic intermediate accompanied by reduced glycolytic capacity.
Overall design We performed combined RNA-Seq, miRNA-seq, and ribosome profiling in shBCDIN3D MDA-MB-231 breast cancer cells
Contributor(s) Reinsborough CW, Ipas H, Abell NS, Gouws EB, Williams JP, Mercado M, Ven Den Berg C, Xhemalçe B
Citation(s) 33664453
Submission date Sep 28, 2020
Last update date Apr 14, 2021
Contact name Blerta Xhemalce
Organization name University of Texas at Austin
Street address 2500 Speedway
City Austin
State/province Texas
ZIP/Postal code 78712
Country USA
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (24)
GSM4805330 RiboProfiling-shNC-RapMinus-Rep1
GSM4805331 RiboProfiling-shNC-RapPlus-Rep1
GSM4805332 RiboProfiling-shBCDIN3D-RapMinus-Rep1
BioProject PRJNA666167
SRA SRP285644

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE158669_RAW.tar 1.5 Gb (http)(custom) TAR (of BW)
GSE158669_RNA-counts.txt.gz 530.0 Kb (ftp)(http) TXT
GSE158669_miRNA-counts.txt.gz 32.6 Kb (ftp)(http) TXT
GSE158669_sRNA-counts.txt.gz 241.0 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap