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| Status |
Public on Sep 30, 2020 |
| Title |
Transcriptome analysis of programmed cell death during allorecognition in Cryphonectria parasitica |
| Organism |
Cryphonectria parasitica |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The underlying molecular mechanisms of programmed cell death (PCD) associated with fungal allorecognition, a form of innate immunity, remain largely unknown. In this study, transcriptome analysis was used to infer mechanisms activated during barrage formation in vic3-incompatible strains of C. parasitica, the chestnut blight fungus. Pronounced differential expression occurred in barraging strains of genes involved in mating pheromone (mf2-1, mf2-2), secondary metabolite production, detoxification (including oxidative stress), apoptosis-related, RNA interference and HET-domain genes. Evidence for secondary metabolite production and reactive oxygen species (ROS) accumulation is supported through UPLC-HRMS analysis and cytological staining, respectively. Differential expression of mating-related genes and HET-domain genes was further examined by RT-qPCR of incompatible interactions involving each of the 6 vegetative incompatibility (vic) loci in C. parasitica and revealed distinct recognition process networks. We infer that vegetative incompatibility in C. parasitica activates defence reactions that involve secondary metabolism, resulting in increased toxicity of the extra- and intracellular environment. Accumulation of ROS (and other potential toxins) may result in detoxification failure and activation of apoptosis, sporulation, and the expression of associated pheromone genes. The incompatible reaction leaves abundant traces of a process-specific metabolome as conidiation is initiated.
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| Overall design |
Identification of genes involved in allorecognition in C. parasitica. Total 15 samples: 5 samples with 3 replicas. Controls: 3 monoculture strains (P74-3, EP155, DZ-66), 1 mix strain sample (P74-3+DZ-66). Experiment: 1 mix strain sample (P74-3+P155).
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| Contributor(s) |
Belov AA, Witte T, Overy DP, Smith ML |
| Citation missing |
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| |
| Submission date |
Sep 29, 2020 |
| Last update date |
Oct 01, 2020 |
| Contact name |
Myron Smith |
| E-mail(s) |
myronsmith@cunet.carleton.ca
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| Organization name |
Carleton University
|
| Department |
Biology
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| Street address |
1125 Colonel By drive
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| City |
Ottawa |
| State/province |
Ontario |
| ZIP/Postal code |
K1S 5B6 |
| Country |
Canada |
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| Platforms (1) |
| GPL29197 |
Illumina NovaSeq 6000 (Cryphonectria parasitica) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA666425 |
| SRA |
SRP285806 |
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