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Series GSE15879 Query DataSets for GSE15879
Status Public on Jul 31, 2010
Title Effects of chronic hypoxia on Duchenne Muscular Dystrophy Drosophila melanogaster model
Organism Drosophila melanogaster
Experiment type Expression profiling by array
Summary In order to understand the chronic hypoxia (CH) effect upon the absence of dystrophin, Drosophila melanogaster wild type and the model for DMD (dmDys), in which all dystrophins expression was knocked out by iRNA, were exposed to high altitude hypoxia (hypobaric hypoxia) during a 16-day climbing period reaching the summit of Mount McKinley (6194 meters above sea level). Furthermore, dmDys and Drosophila wild type were exposed to normobaric hypoxia (hypoxic chamber) following the same oxygen levels observed during the climbing expedition and to normoxic conditions for comparison. Affymetrix GeneChip® profiling was performed for individual flies from each experimental group. CH-dmDys differentially expressed 1281 genes, whereas control group differentially expressed 57 genes. Eight heat shock protein genes detected in the CH-dmDys microarray study were down-regulated, instead of up-regulated as seen in wild type hypoxic flies. This result suggests a differential gene expression response to CH, which could affect muscle performance.These results suggest that dmDys is more sensitive to CH due to reduced muscle function and hypoxic stress response.
 
Overall design In order to understand the chronic hypoxia (CH) effect upon the absence of dystrophin, Drosophila melanogaster wild type and the model for DMD (dmDys), in which all dystrophins expression was knocked out by iRNA, were exposed to high altitude hypoxia (hypobaric hypoxia) during a 16-day climbing period reaching the summit of Mount McKinley (6194 meters above sea level). Furthermore, dmDys and Drosophila wild type were exposed to normobaric hypoxia (hypoxic chamber) following the same oxygen levels observed during the climbing expedition and to normoxic conditions for comparison. Affymetrix GeneChip® profiling was performed for individual flies from each experimental group. CH-dmDys differentially expressed 1281 genes, whereas control group differentially expressed 57 genes. Eight heat shock protein genes detected in the CH-dmDys microarray study were down-regulated, instead of up-regulated as seen in wild type hypoxic flies. This result suggests a differential gene expression response to CH, which could affect muscle performance.These results suggest that dmDys is more sensitive to CH due to reduced muscle function and hypoxic stress response.
Overall design:
Adults wild type and dystrophic flies (3-5 days old) were exposed to hypobaric hypoxia for two weeks during the summer expedition to Mount McKinley, Alaska (6194 MASL). Another set of wild types and dystrophic flies were exposed to normobaric hypoxia according to the table I obtained during the climbing expedition. During the expedition, the flies were maintained in vials with regular molasses and covered by thermo isolation to avoid low temperature, keeping the temperature at 25C. The experiment performed in the laboratory also used vials with regular molasses and at 25C.
Table I. Expedition log book for mount McKinley ascent. Information obtained during the ascent and summit of Mount McKinley, June 1st to June 16th of 2007. The oxygen pressure (PO2) was calculated from the barometric pressure. GNB means go and back from the mentioned point.

DAY LOCATION ALTITUDE
m PO2
mmHg (%)
1 Base Camp 2200 123.6 (16.3%)
2 Base Camp 2200 123.6 (16.3%)
3 Base Camp 2200 123.6 (16.3%)
4 Ski Hill 2400 120.7 (15.9%)
5 Kahiltna Pass 2950 113.0 (14.9%)
6 Motorcycle Hill 3350 107.7 (14.2%)
7 Motorcycle Hill 3350 107.7 (14.2%)
8 GNB from Motorcycle 4150 (5 hours) 97.7 (12.9%)
9 Medical Camp 4350 95.3 (12.5%)
10 GNB from Medical Camp 4150 (5 hours) 97.7 (12.9%)
11 Medical Camp 4350 95.3 (12.5%)
12 GNB from Medical Camp 4900 89.0 (11.7%)
13 Medical Camp 4350 95.3 (12.5%)
14 High Camp 5250 85.1 (11.2%)
15 Summit 6194 (0.3 hours) 75.4 (9.9%)
16 High Camp 5250 85.1 (11.2%)

 
Citation(s) 20975992
Submission date Apr 28, 2009
Last update date Aug 28, 2018
Contact name Matias Mosqueira
E-mail(s) matias@physiologie.uni-heidelberg.de
Phone +496221544143
Organization name Heidelberg University Hospital
Department Institute of Physiology and Pathop
Lab Cardio-Ventilatory Muscle Physiology
Street address Im Neuenheimer Feld, 326 R414
City Heidelberg
State/province Baden-Wuttenberg
ZIP/Postal code 69120
Country Germany
 
Platforms (1)
GPL1322 [Drosophila_2] Affymetrix Drosophila Genome 2.0 Array
Samples (16)
GSM398827 Dystrophic_Drosophila_chronic_hypoxia_sample5
GSM398828 Dystrophic_Drosophila_chronic_hypoxia_sample6
GSM398829 Dystrophic_Drosophila_chronic_hypoxia_sample7
Relations
BioProject PRJNA117037

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE15879_RAW.tar 36.6 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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