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Series GSE158905 Query DataSets for GSE158905
Status Public on Oct 02, 2020
Title Diet and CLK-dependent expression changes in fly heads
Organism Drosophila melanogaster
Experiment type Expression profiling by high throughput sequencing
Summary This study aims to elucidate how the core circadian transcription factor, CLOCK (Clk), mediates diet-dependent transcriptional changes in fly heads. In particular, we sought to identify how CLK influences gene-level expression changes in fly heads from flies that had been reared on either a high nutrient or low nutrient (dietary restriction) diet.
 
Overall design We performed RNA-Seq analyses of heads with inducible, pan-neuronal over-expression of dominant negative CLK protein lacking the basic-helix-loop-helix domain (DNA binding). Flies were heterozygous for the RU486-inducible Elav-GeneSwitch-GAL4 driver as well as a UAS-controlled dominant negative clock allele (CLK-D1). Flies were reared on either a nutrient rich (ad libitum, 5% yeast-extract) or dietary restriction (0.5% yeast-extract) diet with or without the addition of RU486 (200μM) in a 12:12h light-dark cycle at 25°C. mRNA was collected at ZT0 and ZT12 from three independent biological replicates (per genotype/diet) and deep-sequencing was performed (150 PE reads, read depth of 25 million).
 
Contributor(s) Hodge BA, Meyerhof GT, Kapahi P
Citation(s) 35672419
Submission date Oct 01, 2020
Last update date Jun 14, 2022
Contact name Brian A Hodge
E-mail(s) bhodge@buckinstitute.org
Phone 7193737545
Organization name Buck Institute
Lab Kapahi
Street address 338 EDGEWOOD AVE, A
City MILL VALLEY
State/province CA
ZIP/Postal code 94941-2630
Country USA
 
Platforms (1)
GPL25244 Illumina NovaSeq 6000 (Drosophila melanogaster)
Samples (24)
GSM4815628 D.Melanogaster_Elav-GS-Gal4>UAS-CLK-DN1_female_heads_AL-diet_Vehcle-treated_control_ZT0_Replicate-1
GSM4815629 D.Melanogaster_Elav-GS-Gal4>UAS-CLK-DN1_female_heads_AL-diet_Vehcle-treated_control_ZT0_Replicate-2
GSM4815630 D.Melanogaster_Elav-GS-Gal4>UAS-CLK-DN1_female_heads_AL-diet_Vehcle-treated_control_ZT0_Replicate-3
Relations
BioProject PRJNA666923
SRA SRP286060

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE158905_ZT0_AL-cnt_vs_AL-CLK-DN1.txt.gz 1.3 Mb (ftp)(http) TXT
GSE158905_ZT0_AL-cnt_vs_DR-cnt.txt.gz 1.3 Mb (ftp)(http) TXT
GSE158905_ZT0_DR-cnt_vs_DR-CLK-DN1.txt.gz 1.3 Mb (ftp)(http) TXT
GSE158905_ZT12_AL-cnt_vs_AL-CLK-DN1.txt.gz 1.3 Mb (ftp)(http) TXT
GSE158905_ZT12_AL-cnt_vs_DR-cnt.txt.gz 1.3 Mb (ftp)(http) TXT
GSE158905_ZT12_DR-cnt_vs_DR-CLK-DN1.txt.gz 1.4 Mb (ftp)(http) TXT
GSE158905_nCLK-DN1-RNA-Seq_normalized_counts.txt.gz 2.0 Mb (ftp)(http) TXT
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