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| Status |
Public on Mar 31, 2021 |
| Title |
Differential transcriptome of human dendritic cells at first contact with Trypanosoma cruzi reveals response to virus as a central unexplored pathway |
| Organisms |
Trypanosoma cruzi; Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Chagas disease is a notorious public health problem worldwide. However, the pathology is still poorly understood in humans and dendritic cells (DCs) have a determinant role during infection that could help to understand what happens with immune response during the first contact. Here we performed a transcriptomic analysis of the interaction between DCs from three human donors and the infective forms of T. cruzi for 12 h. 468 differentially expressed genes (DEGs) were found in DCs after infection. KEGG and GO enrichment analysis showed that most of DEGs are involved in pathways related to virus infection and intracellular receptors, as NOD and RIG-I -like receptors. Some of these DEGs were validated through RT-qPCR, corroborating the transcriptome data. Cytokine measurement partially corresponded to the transcript levels. In conclusion, this study has revealed the virus response as an unexplored pathway in Chagas disease, which brings new perspectives for this pathology.
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| Overall design |
3 human donors, 2 replicates each, with control and infected samples for each experiment. A total of 12 samples were analyzed.
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| Contributor(s) |
Gil-Jaramillo N, Rocha AP, Raiol T, Motta FN, Favali CB, Brigido MM, Bastos IM, Santana JM |
| Citation(s) |
33897690 |
| |
| Submission date |
Oct 04, 2020 |
| Last update date |
Apr 28, 2021 |
| Contact name |
Taina Raiol |
| E-mail(s) |
taina.raiol@fiocruz.br
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| Organization name |
Oswaldo Cruz Foundation - Fiocruz
|
| Department |
Fiocruz Brasília (GEREB)
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| Street address |
Av. L3 Norte, Campus Universitário Darcy Ribeiro
|
| City |
Brasília |
| State/province |
DF |
| ZIP/Postal code |
70.904-130 |
| Country |
Brazil |
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| Platforms (2) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL29219 |
Illumina HiSeq 2500 (Homo sapiens; Trypanosoma cruzi) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA667258 |
| SRA |
SRP286302 |
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