|
| Status |
Public on Oct 19, 2020 |
| Title |
Interepithelial inflammatory crosstalk mediated by stromal fibroblast communication through hyaluronan [dataset 2] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Inflammation of epithelial structures frequently leads to disease at distal organs, but the mechanism responsible for this is unknown. We report evidence that digestion of hyaluronan in the extracellular matrix signals distal stromal cells to become primed for increased inflammation. By applying transgenic mouse models and single cell RNA sequencing of mouse and human tissues we demonstrate that skin injury or infection promotes an adipogenic response in distinct populations of distant submucosal fibroblasts of the colon. This response can be recapitulated without skin inflammation by expression of hyaluronidase in skin that promotes a highly amplified and frequently fatal host response to intestinal injury. Our results uncover an innate system of communication between epithelial stroma that is not initiated by immunocyte trafficking or cytokine signaling.
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| |
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| Overall design |
RNA-seq of mouse colon lamina propria
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| |
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| Contributor(s) |
Dokoshi T, Gallo RL |
| Citation(s) |
34720087 |
| |
| Submission date |
Oct 04, 2020 |
| Last update date |
Jan 06, 2022 |
| Contact name |
Jason Samuel Seidman |
| E-mail(s) |
jseidman@ucsd.edu
|
| Phone |
6507763250
|
| Organization name |
University of California San Diego
|
| Department |
Cellular and Molecular Medicine
|
| Lab |
Christopher K Glass
|
| Street address |
9500 Gilman Drive, Mail code 0651
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE159509 |
Interepithelial inflammatory crosstalk mediated by stromal fibroblast communication through hyaluronan |
|
| Relations |
| BioProject |
PRJNA667261 |
| SRA |
SRP286305 |
