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| Status |
Public on Jan 13, 2021 |
| Title |
Germline inherited small RNAs facilitate the clearance of untranslated maternal mRNAs in C. elegans embryos (Degradome-seq) |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Other
|
| Summary |
Inheritance and clearance of maternal mRNAs are two of the most critical events required for animal early embryonic development. However, the mechanisms regulating this process are still largely unknown. Here, we show that together with maternal mRNAs, C. elegans embryos inherit a complementary pool of small non-coding RNAs that facilitate the cleavage and removal of hundreds of maternal mRNAs. These antisense small RNAs are loaded into the maternal catalytically-active Argonaute CSR-1 and cleave complementary mRNAs no longer engaged in translation in somatic blastomeres. Induced depletion of CSR-1 specifically during embryonic development leads to embryonic lethality in a slicer-dependent manner and impairs the degradation of CSR-1 embryonic mRNA targets. Given the conservation of Argonaute catalytic activity, we propose that a similar mechanism operates to clear maternal mRNAs during the maternal-to-zygotic transition across species.
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| |
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| Overall design |
4 samples were analyzed by Degradome-seq, including replicates (sample name contains 'rep').
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| |
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| Contributor(s) |
Quarato P, Singh M, Cornes E, Li B, Didier C, Bourdon L, Cecere G |
| Citation(s) |
33664268 |
| |
| Submission date |
Oct 06, 2020 |
| Last update date |
Apr 06, 2021 |
| Contact name |
Germano Cecere |
| E-mail(s) |
germano.cecere@pasteur.fr
|
| Phone |
0033140613225
|
| Organization name |
Institut Pasteur
|
| Department |
Development and stem cell biology
|
| Lab |
Mechanisms of Epigenetic Inheritance
|
| Street address |
Institut Pasteur, 28 Rue Du Docteur Roux, Batiment Monod, 4eme Etage
|
| City |
Paris |
| ZIP/Postal code |
75724 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL19757 |
Illumina NextSeq 500 (Caenorhabditis elegans) |
|
| Samples (4)
|
| GSM4818858 |
CSR-1_degron_CSR-1_rescue_DDH_embryos_rep1_Deg-seq |
| GSM4818859 |
CSR-1_degron_CSR-1_rescue_DDH_embryos_rep2_Deg-seq |
| GSM4818860 |
CSR-1_degron_CSR-1_rescue_ADH_embryos_rep1_Deg-seq |
| GSM4818861 |
CSR-1_degron_CSR-1_rescue_ADH_embryos_rep2_Deg-seq |
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| This SubSeries is part of SuperSeries: |
| GSE146062 |
Germline inherited small RNAs facilitate the clearance of untranslated maternal mRNAs in C. elegans embryos |
|
| Relations |
| BioProject |
PRJNA667657 |
| SRA |
SRP286513 |
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