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| Status |
Public on Oct 07, 2020 |
| Title |
SIX1 and SIX4 homeoproteins regulate PAX7+ progenitor cell properties during fetal epaxial myogenesis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Identification of the gene targets of the SIX transcription factors in myogenic stem cells and in whole back muscles during murine fetal development
Pax7 expression marks stem cells in developing skeletal muscles and adult satellite cells during homeostasis and muscle regeneration. The genetic determinants that control the entrance into the myogenic program and the appearance of PAX7+ cells during embryogenesis are poorly understood. SIX homeoproteins are encoded by the Sine oculis homeobox related Six1-Six6 genes in vertebrates. Six1, Six2, Six4 and Six5 are expressed in the muscle lineage. Here we tested the hypothesis that Six1 and Six4 could participate in the genesis of myogenic stem cells. We show that fewer PAX7+ cells occupy a satellite cell position between the myofiber and its associated basal lamina in Six1 and Six4 (s1s4KO) at E18. However, PAX7+ cells are detected in remaining muscle masses present in the epaxial region of the double mutant embryos and are able to divide and contribute to muscle growth. To further characterize the properties of s1s4KO PAX7+ cells, we analyzed their transcriptome and tested their properties after transplantation in adult regenerating tibialis anterior (TA) muscle. Mutant stem cells form hypotrophic myofibers that are not innervated but retain the ability to self-renew.
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| Overall design |
We analyzed three WT, three SIX1/4 mutant myogenic stem cells at E15.5, and the same at E18.5 (PAX7 samples). We analyzed three SIX1/4 mutant and three WT whole back muscle at E15.5 and two WT and two Six1/4 mutant at E18.5 (Muscle samples).
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| Contributor(s) |
Wurmser M, Maire P |
| Citation missing |
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| |
| Submission date |
Oct 06, 2020 |
| Last update date |
Oct 09, 2020 |
| Contact name |
Pascal Maire |
| E-mail(s) |
pascal.maire@inserm.fr
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| Organization name |
Institut Cochin. INSERM U1016, CNRS UMR 8104, U.Paris
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| Department |
Développement Reproduction et Cancer
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| Street address |
24 Rue du Fg St Jacques
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| City |
PARIS |
| ZIP/Postal code |
75014 |
| Country |
France |
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| Platforms (3) |
| GPL20710 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array (mogene20st_Mm_ENTREZG_19) |
| GPL21883 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [CDF: mogene10st_Mm_ENTREZG_20] |
| GPL22598 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array (mogene20st_Mm_ENTREZG_20.0.0) |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA667661 |
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