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| Status |
Public on Oct 07, 2020 |
| Title |
Transcriptional response of Mycobacterium tuberculosis to inhibition of Cytochrome bd oxidase and Cytochrome bcc:aa3 oxidase |
| Organism |
Mycobacterium tuberculosis |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Comparative analysis of the transcriptional response, as quantified by RNA-Seq, of Mycobacterium tuberculosis (Mtb) during inhibition of the terminal cytochrome oxidases, Cytochrome bd oxidase and Cytochrome bcc:aa3. This study was designed to evaluate the efficacy if a novel small molecule inhibitor of the Cytochrome bd oxidase. Specifically, we compared the transcriptional response of Mtb during inhibition by Q203 with a Cytochrome bd deletion mutant to the transcriptional response of Mtb treated with a combination of Q203 and the purported Cytorchomre bd small molecule inhibitor (ND-011992).
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| Overall design |
Comparison of mRNA transcript levels before and after drug treatment of WT Mycobacterium tuberculosis and Cytochrome bd oxidase mutant (∆CydAB). Measured before and after 6 h after treatment with Q203, ND-011992, or combination. Biological replicates: 3.
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| |
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| Contributor(s) |
Hasenoehrl EJ, Berney M |
| Citation(s) |
33283973 |
| |
| Submission date |
Oct 06, 2020 |
| Last update date |
Jan 08, 2021 |
| Contact name |
Erik James Hasenoehrl |
| E-mail(s) |
erik.hasenoehrl@gmail.com
|
| Organization name |
Albert Einstein College of Medicine
|
| Department |
Microbiology & Immunology
|
| Lab |
Berney Lab
|
| Street address |
1301 Morris Park Ave. Price 569
|
| City |
Bronx |
| State/province |
New York |
| ZIP/Postal code |
10461 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL25498 |
NextSeq 550 (Mycobacterium tuberculosis) |
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| Samples (23)
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| Relations |
| BioProject |
PRJNA667658 |
| SRA |
SRP286514 |
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