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| Status |
Public on Jun 29, 2009 |
| Title |
Expression profiling of Ruminococcus flavefaciens FD-1 with growth on cellulose or cellobiose |
| Organism |
Ruminococcus flavefaciens FD-1 |
| Experiment type |
Expression profiling by array
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| Summary |
This study compares growth of Ruminococcus flavefaciens FD-1 with cellulose or cellobiose as the carbohydrate substrate.
Ruminococcus flavefaciens is a predominant cellulolytic rumen bacterium, which forms a multi-enzyme cellulosome complex that could play an integral role in the ability of this bacterium to degrade plant cell wall polysaccharides. Identifying the major enzyme types involved in plant cell wall degradation is essential for gaining a better understanding of the cellulolytic capabilities of this organism as well as highlighting potential enzymes for application to improvement of livestock nutrition and for conversion of cellulosic biomass to liquid fuels. These results show that the growth substrate drives expression of enzymes predicted to be involved in carbohydrate metabolism as well as expression and assembly of key cellulosomal enzyme components.
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| Overall design |
1 species (Ruminococcus flavefaciens FD_1), 2 conditions (cellulose, cellobiose), 4 biological replicates. Direct design with biological dye swap.
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| Contributor(s) |
Berg Miller ME, Antonopoulos DA, Rincon MT, Band M, Bari A, Akraiko T, Hernandez A, Thimmapuram J, Henrissat B, Coutinho PM, Borovok I, Jindou S, Lamed R, Flint HJ, Bayer EA, White BA |
| Citation(s) |
19680555 |
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| Submission date |
Apr 30, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Bryan A White |
| E-mail(s) |
bwhite44@illinois.edu
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| Phone |
217-333-2091
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| Fax |
217-333-8286
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| Organization name |
University of Illinois
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| Department |
Animal Sciences
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| Street address |
1207 W. Gregory Drive
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| City |
Urbana |
| State/province |
IL |
| ZIP/Postal code |
61801 |
| Country |
USA |
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| Platforms (1) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA116917 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE15916_RAW.tar |
4.4 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
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