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| Status |
Public on Jan 01, 2021 |
| Title |
Influence of 3HP and PDO on cell metabolism at the transcriptional level in Halomonas bluephagenesis |
| Organism |
Halomonas bluephagenesis |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investigate degradation genes in the 3HP degradation pathway, transcriptome sequencing was performed under culture conditions with and without 3HP as the carbon source. Overall, in the presence of 3HP as a sole carbon source, 384 genes were upregulated while 210 downregulated. Among them, we identified two 3HP degradation genes, GME_RS01260 encoding choline dehydrogenase (RefSeq. Accession: WP_009721523) and GME_RS01255 encoding CoA-acylating methylmalonate-semialdehyde dehydrogenase (RefSeq. Accession WP_009721522). Subsequently, transcriptome sequencing was performed under the culture condition with or without addition of PDO as a carbon source. Overall, 292 genes were upregulated while 285 downregulated with the addition of PDO. Among them, we identified an alcohol dehydrogenases AdhP (RefSeq. Accession: WP_039868491.1).
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| Overall design |
We compared the differential gene expression in H. bluephagenesis under culture conditions with and without 3HP/PDO as the carbon source.
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| Contributor(s) |
Jiang X, Chen G |
| Citation(s) |
33686068 |
| |
| Submission date |
Oct 08, 2020 |
| Last update date |
Mar 16, 2021 |
| Contact name |
Xiao-Ran Jiang |
| E-mail(s) |
jiangxiaoranwork@hotmail.com
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| Organization name |
Army Medical University
|
| Department |
Department of Microbiology
|
| Street address |
No.30 Gaotanyan Main Street, Shapingba District
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| City |
Chongqing |
| ZIP/Postal code |
400038 |
| Country |
China |
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| Platforms (1) |
| GPL29233 |
Illumina NovaSeq 6000 (Halomonas bluephagenesis) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA668148 |
| SRA |
SRP286783 |
