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Series GSE159450 Query DataSets for GSE159450
Status Public on Oct 14, 2020
Title A critical role for STING signaling in limiting pathogenesis of Chikungunya virus
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The stimulator-of-interferon-gene (STING) pathway controls both DNA and RNA virus infection. STING is essential for induction of innate immune responses during DNA virus infection, while its mechanism against RNA virus remains largely elusive. We show that STING signaling is crucial for restricting Chikungunya virus infection and arthritis pathogenesis. Sting-deficient mice (Stinggt/gt) had elevated viremia throughout the viremic stage and viral burden in the feet transiently, along with a normal type I IFN response. Stinggt/gt mice presented much greater foot swelling, joint damage and immune cell infiltration than WT mice. Intriguingly, expression of interferon gamma and its induced Cxcl10 was continuously upregulated by ~7-10-fold, and further elevated in Stinggt/gt mice synchronously with arthritis progression. However, expression of chemoattractants for and activators of neutrophils, Cxcl5, Cxcl7 and Cxcr2 was suppressed in Stinggt/gt joints. These results demonstrate that STING deficiency leads to an aberrant chemokine response that promotes pathogenesis of CHIKV arthritis.
 
Overall design Bone-marrow-derived macrophages from three individual WT/Sting gt/gt mice were infected by CHIKV at a multiplicity of infection of 5 for 12 hrs or not infected (mock). The whole genome transcriptome was analyzed by RNAseq.
 
Contributor(s) Geng T, Wang P
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Submission date Oct 13, 2020
Last update date Oct 20, 2020
Contact name Tingting Geng
E-mail(s) geng@uchc.com
Organization name Uconn Health
Department Department of Immunology
Lab Penghua Wang
Street address 263 Farmington Avenue
City Farmington
State/province CT
ZIP/Postal code 06030
Country USA
 
Platforms (1)
GPL23479 BGISEQ-500 (Mus musculus)
Samples (12)
GSM4829990 WT1_0h
GSM4829991 WT2_0h
GSM4829992 WT3_0h
Relations
BioProject PRJNA668984
SRA SRP287249

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE159450_GK_0h-VS-GK_12h.DEGseq_Method.GeneDiffExp.txt.gz 2.9 Mb (ftp)(http) TXT
GSE159450_Suppl-Table1-DEG-List.xlsx 9.7 Mb (ftp)(http) XLSX
GSE159450_WT_0h-VS-GK_0h.DEGseq_Method.GeneDiffExp.txt.gz 2.9 Mb (ftp)(http) TXT
GSE159450_WT_0h-VS-WT_12h.DEGseq_Method.GeneDiffExp.txt.gz 3.0 Mb (ftp)(http) TXT
GSE159450_WT_12h-VS-GK_12h.DEGseq_Method.GeneDiffExp.txt.gz 2.9 Mb (ftp)(http) TXT
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