|
| Status |
Public on Jun 30, 2009 |
| Title |
Down-regulation of SFRP1 in a mammary epithelial cell line |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Microarray data to accompany Gauger et al. (2009) Cancer Cell International paper
|
| |
|
| Overall design |
A treated X untreated design was used whereby immortalized human mammary epithelial cell lines (TERT cells) were modified to stably express an siRNA that knocked down SFRP1 expression. These "treated" cells were labeled with Cy5 and four replicate harvests were hybridized to Agilent 4by44k whole genome microarrays, along with a Cy3 labeled untreated control. The untreated control consisted of 4 pooled harvests of the parent cell line in normal growth conditions at 60-80% confluence.
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| |
|
| Citation(s) |
19422694 |
| |
| Submission date |
May 04, 2009 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Melissa Troester |
| E-mail(s) |
troester@unc.edu
|
| Organization name |
University of North Carolina at Chapel Hill
|
| Department |
Epidemiology
|
| Street address |
135 Dauer Drive, CB 7435
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA117005 |
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