|
| Status |
Public on May 05, 2009 |
| Title |
Hypoxia inducible factors 1 and 2 are important transcriptional effectors in primary macrophages experiencing hypoxia |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Ischemia exists in many diseased tissues including arthritic joints, atherosclerotic plaques and malignant tumors. Macrophages accumulate in these sites and upregulate genes in response to the hypoxia present.
We used microarrays to detail the hypoxia upregulated gene in human primary macrophages.
|
| |
|
| Overall design |
Primary macrophages were differentiated for 7 days in vitro from human peripheral blood (monocyte-derived macrophages, MDMs) and were subjected to severe hypoxia (< 0.5% O2) or normoxia (20.9% O2) in 5% CO2 humidified multi-gas incubators.
|
| |
|
| Contributor(s) |
Fang H, Heath P |
| Citation(s) |
19454749 |
| |
| Submission date |
May 04, 2009 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Hsin-Yu Fang |
| E-mail(s) |
mdp05hf@sheffield.ac.uk
|
| Organization name |
University of Sheffield
|
| Department |
Infection & Immunity,School of Medicine & BioMedical Sciences
|
| Lab |
Tumour Targeting Group
|
| Street address |
Beech Hill Rd
|
| City |
sheffield |
| ZIP/Postal code |
S10 2RX |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA117007 |
