|
| Status |
Public on Mar 11, 2024 |
| Title |
Molecular analysis of the subaortic hematopoietic stem cell niche [E11.5] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Hematopoietic stem cells (HSCs) are formed in the Aorta-Gonad-Mesobephros (AGM) region of mouse midgestation embryos. This process is tightly regulated in time and space by surrounding cells inculding mesenchymal cells located underneath the dorsal aorta.
We combined laser microdissection and microarrays to isolate the dorsal and ventral aortic tissues at three developmental stages and to explore their gene expression profiling.
|
| |
|
| Overall design |
The dorsal and ventral aortic tissues (located in between the notochord and the roof of the dorsal aorta and underneath the dorsal aorta, respectively) were isolated by laser microdissection in the mouse embryo at three stages of AGM hematopoiesis (E10.5, E11.5 and E12.5). Microdissected tissues were collected on appropriate caps and then proceeded for RNA extraction using Acturus Picopure RNA extraction kit and Affymetrix microarrays mouse Gene 2.0 ST.
|
| |
|
| Contributor(s) |
Durand C, Charbord P, Jaffredo T |
| Citation(s) |
38451068 |
| |
| Submission date |
Oct 19, 2020 |
| Last update date |
Jun 18, 2024 |
| Contact name |
Charles Durand |
| E-mail(s) |
charles.durand@sorbonne-universite.fr
|
| Phone |
+33 1 44 27 22 84
|
| Organization name |
Sorbonne University
|
| Department |
Laboratory of Developmental Biology
|
| Lab |
CNRS UMR7622
|
| Street address |
9, quai saint-Bernard
|
| City |
Paris |
| ZIP/Postal code |
75005 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL20710 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array (mogene20st_Mm_ENTREZG_19) |
|
| Samples (6)
|
| GSM4832859 |
Dorsal tissue at E11.5, biological rep 1 |
| GSM4832860 |
Dorsal tissue at E11.5, biological rep 2 |
| GSM4832861 |
Dorsal tissue at E11.5, biological rep 3 |
| GSM4832862 |
Ventral tissue at E11.5, biological rep 1 |
| GSM4832863 |
Ventral tissue at E11.5, biological rep 2 |
| GSM4832864 |
Ventral tissue at E11.5, biological rep 3 |
|
| This SubSeries is part of SuperSeries: |
| GSE159592 |
Molecular analysis of the subaortic hematopoietic stem cell niche |
|
| Relations |
| BioProject |
PRJNA670009 |
Less...
More...