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| Status |
Public on Dec 01, 2021 |
| Title |
Ovol1 regulates psoriasis-like skin inflammation and epidermal hyperplasia |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Psoriasis is a common inflammatory skin disease characterized by aberrant inflammation and epidermal hyperplasia. Molecular mechanisms that regulate psoriasis-like skin inflammation remain to be fully understood. Here we show that the expression of Ovol1 transcription factor is upregulated in psoriatic skin, and its deletion results in aggravated psoriasis-like skin symptoms following stimulation with imiquimod (IMQ). Using bulk and single-cell RNA-sequencing, we identify molecular changes in the epidermal, fibroblast and immune cells of Ovol1-deficient skin that reflect altered course of epidermal differentiation and enhanced inflammatory responses. Furthermore, we provide evidence for excessive full-length IL-1 signaling in the microenvironment of IMQ-treated Ovol1-deficient skin that functionally contributes to immune cell infiltration and epidermal hyperplasia. Collectively, our study uncovers a protective role for Ovol1 in curtailing psoriasis-like inflammation and the associated skin pathology
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| Overall design |
Droplet-based single cell RNA-seq experiments were performed to identify molecular differences between control and Ovol1 null in untreated and imiquimod (IMQ) treated adult murine back skin.
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| Contributor(s) |
Dai X, Sun P, Vu R, Gutierrez G |
| Citation(s) |
33333123 |
| |
| Submission date |
Oct 20, 2020 |
| Last update date |
Feb 10, 2022 |
| Contact name |
Remy Vu |
| E-mail(s) |
phuongnv@uci.edu
|
| Organization name |
University of California, Irvine
|
| Street address |
D288 Med Sci I Health Sciences Rd
|
| City |
Irvine |
| ZIP/Postal code |
92697 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA670225 |
| SRA |
SRP287854 |
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