|
| Status |
Public on Mar 11, 2021 |
| Title |
ChAP-seq analysis to identify GoxR binding sites |
| Organism |
Gluconobacter oxydans |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Gene expression in the obligatory aerobic acetic acid bacterium Gluconobacter oxydans was shown to respond to oxygen limitation, but the regulators involved are unknown. In this study, we analyzed the function of a transcriptional regulator named GoxR, which belongs to the FNR family. Here, we applied ChAP-seq analysis with a strep-tagged GoxR version to identify binding sites of this regulator in the genome of G. oxydans.
|
| |
|
| Overall design |
For ChAP-seq the strain G. oxydans 621H::goxR-strep was constructed. This strain was cultivated aerobically. After harvesting of the cells, the pellet was treated with formaldehyde to perform cross-linking between bound proteins and the DNA. Afterwards, DNA bound to Strep-tagged GoxR was purified and submitted to sequencing.
|
| |
|
| Contributor(s) |
Kranz A, Bott M |
| Citation(s) |
33741613 |
| |
| Submission date |
Oct 21, 2020 |
| Last update date |
Jun 10, 2021 |
| Contact name |
Angela Kranz |
| E-mail(s) |
a.kranz@fz-juelich.de
|
| Organization name |
Forschungszentrum Jülich GmbH
|
| Department |
IBG-1: Biotechnology
|
| Street address |
Wilhelm-Johnen-Strasse
|
| City |
Jülich |
| State/province |
NRW |
| ZIP/Postal code |
52425 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL28332 |
Illumina MiSeq (Gluconobacter oxydans) |
|
| Samples (1) |
|
| Relations |
| BioProject |
PRJNA670347 |
| SRA |
SRP287951 |
