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| Status |
Public on Oct 24, 2020 |
| Title |
Cold and b3Adr-agonism lead to distinct immunological shifts during beige remodeling of white adipose tissue |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
White adipose tissue (WAT) is a dynamic tissue, which responds to environmental stimuli and dietary cues by changing its morphology and metabolic capacity. The ability of WAT to undergo a beige remodeling has become an appealing strategy to combat obesity and its comorbidities. Here, by using single cell RNA sequencing, we provide a comprehensive atlas of the cellular dynamics during beige remodeling. We reveal drastic changes both in the overall cellular composition and transcriptional states of individual cell subtypes between Adrb3- and cold-induced beiging. Moreover, we demonstrate that cold induces a myeloid to lymphoid shift of the immune compartment compared to Adrb3 activation. Further analysis, showed that Adrb3 stimulation leads to activation of the interferon/Stat1 pathways favoring infiltration of myeloid immune cells, while repression of this pathway by cold promotes lymphoid immune cells recruitment. These findings highlight that pharmacological mimetics may not provide the same beneficial results as physiological stimuli.
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| Overall design |
Single cell of the stromal vascular fraction from mice inguinal adipose tissue foloowing 3 days beta 3 adrenergic or cold exposure.
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| Contributor(s) |
Rabhi N, Belkina AC, Desevin K, Cortez BN, Farmer SR |
| Citation(s) |
33294778 |
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| Submission date |
Oct 23, 2020 |
| Last update date |
Dec 15, 2020 |
| Contact name |
Nabil Rabhi |
| E-mail(s) |
rabhi@bu.edu
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| Phone |
8572659274
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| Organization name |
Boston University
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| Department |
Biochemistry
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| Lab |
Farmer Lab
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| Street address |
Silvio Conte Building, 72 E. Concord St
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| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02118 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA670963 |
| SRA |
SRP288357 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE159966_RAW.tar |
95.8 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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