|
Status |
Public on Jan 11, 2022 |
Title |
Unbiased identification of CSDE1-regulated targets during oncogene-induced senescence [RNA-Seq-sh] |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
|
Summary |
We show that the RNA-binding protein CSDE1/UNR promotes oncogene-induced senescence (OIS) in primary mouse keratynocytes challenged by over-expression of H-RASv12. Depletion of CSDE1 leads to senescence bypass, cell immortalization and tumor formation. Combining individual nucleotide cross-linking and immunoprecipitation (iCLIP), RNA-seq and polysome profiling followed by functional studies, we identify targets regulated by CSDE1 and uncover the downstream molecular mechanisms.
|
|
|
Overall design |
RNA-seq from H-Ras infected PMK upon CSDE1 depletion was performed to identify the CSDE1 targets regulated at the steady state level during OIS.
|
|
|
Contributor(s) |
Avolio R, Gebauer F |
Citation(s) |
35021076 |
|
Submission date |
Oct 23, 2020 |
Last update date |
Apr 19, 2022 |
Contact name |
Fatima Gebauer |
E-mail(s) |
fatima.gebauer@crg.eu
|
Organization name |
Centre for Genomic Regulation (CRG)
|
Department |
Gene Regulation, Stem cells and Cancer Programme
|
Lab |
Regulation of Protein Synthesis in Eukaryotes
|
Street address |
C/Dr. Aiguader, 88
|
City |
Barcelona |
ZIP/Postal code |
08003 |
Country |
Spain |
|
|
Platforms (1) |
GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
Samples (15)
|
GSM4852340 |
sh-UNR 12dpi, replicate 1 |
GSM4852341 |
sh-ctrl 7dpi, replicate 2 |
GSM4852342 |
sh-UNR 7dpi, replicate 2 |
GSM4852343 |
sh-ctrl 12dpi, replicate 2 |
GSM4852344 |
sh-UNR 12dpi, replicate 2 |
GSM4852345 |
sh-ctrl 7dpi, replicate 3 |
GSM4852346 |
sh-UNR 7dpi, replicate 3 |
GSM4852347 |
sh-ctrl 12dpi, replicate 3 |
GSM4852348 |
sh-UNR 12dpi, replicate 3 |
GSM4852349 |
immortalized, replicate 1 |
GSM4852350 |
immortalized, replicate 2 |
GSM4852351 |
immortalized, replicate 3 |
|
This SubSeries is part of SuperSeries: |
GSE160002 |
Unbiased identification of CSDE1-regulated targets during oncogene-induced senescence |
|
Relations |
BioProject |
PRJNA671505 |
SRA |
SRP288392 |