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| Status |
Public on Jan 11, 2022 |
| Title |
Unbiased identification of CSDE1-regulated targets during oncogene-induced senescence [RNA-Seq-sh] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We show that the RNA-binding protein CSDE1/UNR promotes oncogene-induced senescence (OIS) in primary mouse keratynocytes challenged by over-expression of H-RASv12. Depletion of CSDE1 leads to senescence bypass, cell immortalization and tumor formation. Combining individual nucleotide cross-linking and immunoprecipitation (iCLIP), RNA-seq and polysome profiling followed by functional studies, we identify targets regulated by CSDE1 and uncover the downstream molecular mechanisms.
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| |
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| Overall design |
RNA-seq from H-Ras infected PMK upon CSDE1 depletion was performed to identify the CSDE1 targets regulated at the steady state level during OIS.
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| Contributor(s) |
Avolio R, Gebauer F |
| Citation(s) |
35021076 |
| |
| Submission date |
Oct 23, 2020 |
| Last update date |
Apr 19, 2022 |
| Contact name |
Fatima Gebauer |
| E-mail(s) |
fatima.gebauer@crg.eu
|
| Organization name |
Centre for Genomic Regulation (CRG)
|
| Department |
Gene Regulation, Stem cells and Cancer Programme
|
| Lab |
Regulation of Protein Synthesis in Eukaryotes
|
| Street address |
C/Dr. Aiguader, 88
|
| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (15)
|
| GSM4852340 |
sh-UNR 12dpi, replicate 1 |
| GSM4852341 |
sh-ctrl 7dpi, replicate 2 |
| GSM4852342 |
sh-UNR 7dpi, replicate 2 |
| GSM4852343 |
sh-ctrl 12dpi, replicate 2 |
| GSM4852344 |
sh-UNR 12dpi, replicate 2 |
| GSM4852345 |
sh-ctrl 7dpi, replicate 3 |
| GSM4852346 |
sh-UNR 7dpi, replicate 3 |
| GSM4852347 |
sh-ctrl 12dpi, replicate 3 |
| GSM4852348 |
sh-UNR 12dpi, replicate 3 |
| GSM4852349 |
immortalized, replicate 1 |
| GSM4852350 |
immortalized, replicate 2 |
| GSM4852351 |
immortalized, replicate 3 |
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| This SubSeries is part of SuperSeries: |
| GSE160002 |
Unbiased identification of CSDE1-regulated targets during oncogene-induced senescence |
|
| Relations |
| BioProject |
PRJNA671505 |
| SRA |
SRP288392 |
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