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| Status |
Public on Jan 01, 2021 |
| Title |
Long-read RNA sequencing of cattle, pig, and chicken tissues |
| Organisms |
Gallus gallus; Sus scrofa; Bos taurus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Long-read Nanopore cDNA sequencing of polyA-enriched RNA was implemented in a range of adult tissues isolated from cattle, pig, and chicken. These data were used to identify and characterize the expression patterns of full-length transcript isoforms.
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| Overall design |
RNA was extracted from a total of 189 biological samples, corresponding to 93 cattle samples (32 tissues from two males and two females; represented as one 'Cattle_tissues_mixed' sample), 28 pig samples (17 tissues from two males), and 68 chicken samples (19 tissues from four males and two females). Raw data were procured from two lanes of PromethION sequencing: one lane for pooled cattle libraries, and one lane for pooled pig and chicken libraries.
Please note that 'Cattle_tissues_mixed' sample represents non-demultiplexed cattle samples.
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| Contributor(s) |
Halstead MM, Ross PJ |
| Citation(s) |
34093657 |
| |
| Submission date |
Oct 25, 2020 |
| Last update date |
Mar 30, 2022 |
| Contact name |
Michelle M Halstead |
| E-mail(s) |
mhalstead226@gmail.com
|
| Organization name |
University of California, Davis
|
| Department |
Animal Science
|
| Lab |
Ross Lab
|
| Street address |
Meyer Hall, 450 Bioletti Way
|
| City |
Davis |
| State/province |
CA |
| ZIP/Postal code |
95616 |
| Country |
USA |
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| Platforms (3) |
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| Samples (190)
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| Relations |
| BioProject |
PRJNA671673 |
| SRA |
SRP288472 |
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